Community composition of endophytic fungi in Acer truncatum and their role in decomposition
Community composition of endophytic fungi in Acer truncatum and their role in decomposition
复制标题
元宝枫内生真菌群落组成及其分解作用
DOI：
10.1007/s13225-010-0086-5
复制
发表时间：
2011-03-01
期刊：
影响因子：
20.3
通讯作者：
Hyde, K. D.
中科院分区：
文献类型：
--
作者：
Sun, Xiang;Guo, Liang-Dong;Hyde, K. D.
对北方常见树种元宝枫内生真菌的种类及分解潜力进行了研究。内生真菌在枝条中的定殖率（77%）显著高于叶片（11%）。叶片和中脉组织中内生真菌的定殖率分别为9%和14%，差异不显著。共58个内生分类群回收使用两种分离方法，并根据形态学和ITS序列数据进行了鉴定。高数量的叶片内生菌中获得的方法，以确定自然内生菌群落（35分类群）相比，磁盘碎片方法（9分类群）的分解叶。A.元宝草叶片被内生菌群落分解后的失重率随培养时间的延长而增加，20周时的失重率显著高于3周和8周时。常见和罕见的内生类群在体外产生胞外酶，并表现出不同的叶片腐烂能力。结果表明，两种分离方法所得内生真菌的组成和多样性不同。本研究表明，内生真菌在自然生态系统的养分循环中起着重要作用。
The mycota and decomposing potential of endophytic fungi associated with Acer truncatum, a common tree in northern China, were investigated. The colonization rate of endophytic fungi was significantly higher in twigs (77%) than in leaves (11%). However, there was no significant difference in the colonization rates of endophytic fungi between lamina (9%) and midrib (14%) tissues. A total of 58 endophytic taxa were recovered using two isolation methods and these were identified based on morphology and ITS sequence data. High numbers of leaf endophytes were obtained in the method to determine decomposition of leaves by the natural endophyte community (35 taxa) as compared to disk fragment methodology (9 taxa). The weight loss in A. truncatum leaves decomposed by endophyte communities increased with incubation time; the weight loss was significantly higher at 20 weeks than at 3 and 8 weeks. Both common and rare endophytic taxa produced extracellular enzymes in vitro and showed different leaf decay abilities. Our results indicated that the composition and diversity of endophytic fungi obtained differed using two isolation methods. This study suggests that endophytic fungi play an important role in recycling of nutrients in natural ecosystems.