Construction and characterization of an infectious cDNA clone of Echovirus 25
Construction and characterization of an infectious cDNA clone of Echovirus 25
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艾可病毒 25 感染性 cDNA 克隆的构建和表征。
DOI：
10.1016/j.virusres.2015.05.011
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发表时间：
2015-07-02
期刊：
影响因子：
5
通讯作者：
Xia, Ningshao
中科院分区：
文献类型：
--
作者：
Hou, Wangheng;Yang, Lisheng;Xia, Ningshao
埃可病毒25（E-25）是肠道病毒家族的一员，是引起手足口病（HFMD）、脑膜炎、皮疹和呼吸道疾病的常见病原体。本研究从厦门分离的第一株D 6亚型毒株XM 0297中克隆了E-25全长cDNA，并对其进行了鉴定。该株E-25（XM 0297）的5 '-非翻译区（5'-UTR）、P3（3A-3B，3D）和P3（3C）区分别与EV-B77、E-16和E-13高度相似。我们的数据表明，拯救的E-25病毒表现出与原型病毒株XM 0297相似的生长动力学。我们使用透射电子显微镜（TEM）观察了拯救的病毒颗粒，发现它们具有二十面体结构，直径约为30 nm。交叉中和试验表明，E-25（XM 0297）株免疫血清不能中和EV-A71、CV-A16和CV-B3;同样，EV-A71和CV-A16免疫血清也不能中和E-25（XM 0297）。这种感染性克隆的可用性将大大增强未来的病毒学研究和可能的疫苗开发针对E-25。(C)2015 Elsevier B. V.版权所有。
Echovirus 25 (E-25) is a member of the enterovirus family and a common pathogen that induces hand, foot, and mouth disease (HFMD), meningitis, skin rash, and respiratory illnesses. In this study, we constructed and characterized an infectious full-length E-25 cDNA clone derived from the XM0297 strain, which was the first subgenotype D6 strain isolated in Xiamen, China. The 5'-Untranslated Regions (5'-UTR), P3 (3A-3B, 3D) and P3 (3C) regions of this E-25 (XM0297) strain were highly similar to EV-B77, E-16 and E-13, respectively. Our data demonstrate that the rescued E-25 viruses exhibited similar growth kinetics to the prototype virus strain XM0297. We observed the rescued viral particles using transmission electron microscope (TEM) and found them to possess an icosahedral structure, with a diameter of approximately 30 nm. The cross neutralization test demonstrated that the E-25 (XM0297) strain immune serum could not neutralize EV-A71, CV-A16 or CV-B3; likewise, the EV-A71 and CV-A16 immune serum could not neutralize E-25 (XM0297). The availability of this infectious clone will greatly enhance future virological investigations and possible vaccine development against E-25. (C) 2015 Elsevier B.V. All rights reserved.