The hypocholesterolemic activity of transgenic rice seed accumulating lactostatin, a bioactive peptide derived from bovine milk β-lactoglobulin.
The hypocholesterolemic activity of transgenic rice seed accumulating lactostatin, a bioactive peptide derived from bovine milk β-lactoglobulin.
复制标题
转基因水稻种子积累的乳抑素（一种源自牛乳β-乳球蛋白的生物活性肽）的降胆固醇活性。
DOI：
10.1021/jf200044j
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发表时间：
2011
影响因子：
6.1
通讯作者：
F. Takaiwa
中科院分区：
文献类型：
--
作者：
Y. Wakasa;C. Tamakoshi;Tomoki Ohno;S. Hirose;T. Goto;S. Nagaoka;F. Takaiwa
Lactostatin is a novel pentapeptide (IIAEK) derived from bovine milk β-lactoglobulin with greater hypocholesterolemic activity than β-sitosterol, the drug commonly used to treat hypercholesterolemia. We developed transgenic rice expressing lactostatin as a fusion protein with seed storage protein (SSP) glutelins under the control of three different endosperm-specific promoters. Lactostatin accumulated in transgenic rice seed at approximately 1.6 mg/g seeds (dry seeds) without any apparent influence on seed traits such as endogenous SSP expression levels or alterations in the intracellular structures of endosperm cells. Short-term (three day) oral administration of the glutelin fraction containing lactostatin (namely three times of 300 mg/kg body weight/day) extracted from transgenic rice seeds resulted in hypocholesterolemic activity in rats; namely, the serum low-density-lipoprotein (LDL) cholesterol level was significantly reduced accompanied by a significant increase in beneficial serum high-density-lipoprotein (HDL) cholesterol.
影响因子：
4.8
作者：
Usui, Y;Nakase, M;Matsuda, T
通讯作者：
Matsuda, T