Human plasma and tissue α-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E
Human plasma and tissue α-tocopherol concentrations in response to supplementation with deuterated natural and synthetic vitamin E
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DOI：
10.1093/ajcn/67.4.669
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发表时间：
1998-04-01
影响因子：
7.1
通讯作者：
Ingold, KU
中科院分区：
文献类型：
--
作者：
Burton, GW;Traber, MG;Ingold, KU
我们报告了天然和合成维生素E在人使用氘标记允许两种形式的维生素E在血浆和组织中独立测量每个受试者的比较。在相同剂量条件下，使用稀化的rrr - α -生育酚乙酸酯和all-rac- α -生育酚乙酸酯的等摩尔混合物直接测量天然和合成维生素E浓度的差异。两组5名成年人分别服用30毫克的混合物作为单次剂量和连续8次每日剂量。间隔1个月后，重复该计划，但剂量增加了10倍（即300毫克）。在每种情况下，血浆d(3)-RRR- α -生育酚与d(6)-all-rac- α -生育酚（RRR:rac）的比值在给药结束后从大约1.5-1.8增加到大约2。在一项选择性手术研究中，22名患者在手术前给予150mg /d，最多41d，组织中的RRR:rac低于血浆中的RRR:rac，除胆囊和肝脏外，所有组织中氘化醇-生育酚的百分比都较低。在一名绝症患者中，每天服用30mg，持续361天，尸检时血浆和组织（(x)超过bar +/- SD） RRR-rac比率（和%氘化α -生育酚）分别为2.06（6.3%）和1.71 +/- 0.24（5.9 +/- 2.2%）。另一名晚期患者给予300 mg/d，持续615 d，相应值分别为2.11（68%）和2.01 +/- 0.17（65 +/- 10%）。结果表明，天然维生素E的可用性大约是合成维生素E的两倍，这一2:1的比例显著高于目前公认的1.36:1.00的RRR:rac。在15例择期手术患者中，γ -生育酚占未标记生育酚总量的比例，在脂肪组织、肌肉、皮肤和静脉中比在血浆中高1.4-4.6倍（平均：2.6倍），这一比例比之前认识到的要大得多。
We report a comparison of natural and syn thetic vitamin E in humans using deuterium labeling to permit the two forms of vitamin E to be measured independently in plasma and tissues of each subject. Differences in natural and synthetic vitamin E concentrations were measured directly under equal dosage conditions using an equimolar mixture of deuterated RRR-alpha-tocopheryl acetate and all-rac-alpha-tocopheryl acetate. Two groups of five adults took 30 mg of the mixture as a single dose and as eight consecutive daily doses, respectively. After a 1-mo interval the schedule was repeated but with a 10-fold higher dose (ie, 300 mg). In each case, the ratio of plasma d(3)-RRR-alpha-tocopherol to d(6)-all-rac-alpha-tocopherol (RRR:rac) increased from approximate to 1.5-1.8 to approximate to 2 after dosing ended. In an elective surgery study in which 22 patients were given 150 mg/d for up to 41 d before surgery, the RRR:rac in tissues was lower than in plasma and the percentage of deuterated ol-tocopherol was lower in all tissues except gallbladder and liver. In a terminally ill patient given 30 mg/d for 361 d, plasma and tissue ((x) over bar +/- SD) RRR-rac ratios (and % deuterated alpha-tocopherol) at autopsy were 2.06 (6.3%) and 1.71 +/- 0.24 (5.9 +/- 2.2%), respectively. In a second terminally ill patient given 300 mg/d for 615 d, the corresponding values were 2.11 (68%) and 2.01 +/- 0.17 (65 +/- 10%), respectively. The results indicated that natural vitamin E has roughly twice the availability of synthetic vitamin E. This 2:1 ratio is significantly higher than the currently accepted RRR:rac of 1.36:1.00. gamma-Tocopherol, expressed as a fraction of total unlabeled tocopherols in 15 elective surgery patients, was 1.4-4.6 (mean: 2.6) times greater in adipose tissue, muscle, skin, and vein than in plasma, which is a substantially larger fraction than had been recognized previously.