Effects of melittin on molecular dynamics and Ca-ATPase activity in sarcoplasmic reticulum membranes: electron paramagnetic resonance.
Effects of melittin on molecular dynamics and Ca-ATPase activity in sarcoplasmic reticulum membranes: electron paramagnetic resonance.
复制标题
蜂毒肽对肌浆网膜分子动力学和 Ca-ATP 酶活性的影响：电子顺磁共振。
DOI：
10.1021/bi00243a019
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发表时间：
1991
期刊：
影响因子：
2.9
通讯作者：
Thomas,DD
中科院分区：
文献类型：
--
作者：
Mahaney,JE;Thomas,DD
材料和方法试剂和溶液。所有自旋标记物均获自Aldrich。ATP、MOPS和蜂毒肽得自Sigma。所有其他试剂均获自Mallinckrodt，纯度最高。除非另有说明，否则所有酶测定和EPR实验均在25 ℃下在含有60mMKCl、6mMMgCl 2、0.1mMCaCl 2和20mMMOPS的缓冲液（pH 7.0）（此后表示为正常离子强度）或含有0.5M LiCl的相同缓冲液（此后表示为高离子强度）中进行。
Materials and MethodsReagents and Solutions. All spin labels were obtained from Aldrich. ATP, MOPS, and melittin were obtained fromSigma. All other reagents were obtained fromMallinckrodt and were of the highest purity available. All enzymatic assays and EPR experiments were carried out at 25 C in a buffer containing either 60 mM KC1, 6 mM MgCl2, 0.1 mM CaCl2, and 20 mM MOPS, pH 7.0 (henceforth denoted as normal ionic strength), or the same buffer containing 0.5 M LiCl (henceforth denoted as high ionic strength), unless otherwise stated.