Analyses of Reactive Oxygen Species and Antioxidants in Relation to Seed Longevity and Germination
Analyses of Reactive Oxygen Species and Antioxidants in Relation to Seed Longevity and Germination
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DOI：
10.1007/978-1-61779-231-1_20
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Kranner, Ilse
中科院分区：
文献类型：
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作者：
Bailly, Christophe;Kranner, Ilse
有证据表明，活性氧（ROS）和抗氧化剂，以及植物激素和其他活性物质，如活性氮，是有关植物胁迫反应，细胞分裂和细胞死亡的信号网络的一部分。因此，它们在调节种子发育和成熟、萌发和休眠、幼苗建立和种子老化中起关键作用。重要的是，ROS，虽然基本上需要在低浓度下，必须保持在抗氧化剂的严格控制下。如果促氧化和抗氧化过程之间的平衡丧失，ROS产生占主导地位，则氧化应激是结果，其可以诱导细胞死亡并最终导致种子死亡。本章提供了各种协议的测定活性氧，抗氧化剂，和应力标志物，旨在使读者能够量化这些化合物。还描述了可视化ROS和定位ROS产生的位点的协议，希望刺激更多的研究ROS信号和抗氧化剂控制在种子中的关键生理和生化过程。
Evidence is emerging that reactive oxygen species (ROS) and antioxidants, together with plant hormones and other reactive species, such as reactive nitrogen species, arc part of signalling networks pertinent to plant stress responses, cell division, and cell death. Consequently, they play pivotal roles in the regulation of seed development and maturation, germination and dormancy, seedling establishment, and seed ageing. Importantly, ROS, although essentially required at low concentrations, must be kept under stringent control by antioxidants. If the balance between pro- and antioxidative processes is lost and ROS production prevails, oxidative stress is the result, which can induce cell death and ultimately seed death. This chapter offers a variety of protocols for the determination of ROS, antioxidants, and stress markers aimed at enabling the reader to quantify these compounds. Protocols are also described to visualize ROS and localize the sites of ROS production, hoping to stimulate more research into ROS signalling and antioxidant control in key physiological and biochemical processes in seeds.