New monoclonal antibodies against a recombinant second envelope protein of hepatitis C virus
New monoclonal antibodies against a recombinant second envelope protein of hepatitis C virus
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DOI：
10.1111/j.1348-0421.1998.tb02364.x
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发表时间：
1998-01-01
影响因子：
2.6
通讯作者：
Tanaka, Y
中科院分区：
文献类型：
--
作者：
Inudoh, M;Kato, N;Tanaka, Y
为研究丙型肝炎病毒（HCV）包膜蛋白（E2蛋白）的免疫学特性，制备了新的特异性单克隆抗体（mAb）。用表达E2蛋白的牛痘病毒感染的同系细胞和用基于质粒的表达系统从中国仓鼠卵巢细胞获得的可溶性E2蛋白免疫WKA/H大鼠。从免疫大鼠脾细胞中筛选杂交瘤细胞，获得3株特异性单克隆抗体。其中一株单抗与E2蛋白高变区1（HVR 1）的一个肽段反应，其余的单抗与HVR 1以外的区域反应。本文将讨论这些单抗对HCV感染诊断和HCV E2蛋白结构分析的意义。
To study the immunological features of the hepatitis C virus (HCV) envelope protein (E2 protein), new specific monoclonal antibodies (mAbs) were generated. WKA/H rats were immunized with syngeneic cells infected with a vaccinia virus expressing the E2 protein and with soluble E2 protein obtained from Chinese hamster ovary cells with a plasmid-based expression system. By screening hybridoma cells obtained from spleen cells of the immunized rats, three specific mAbs were obtained. One mAb was reactive to a peptide corresponding to the hypervariable region 1 (HVR1) in E2 protein, white the others reacted to regions outside HVR1, The significance of these antibodies for the diagnosis of HCV infection as well as for analysis of the structure of the HCV E2 protein will be discussed.