Characterization of the reaction of decoupling ubiquinone with bovine mitochondrial respiratory complex I
Characterization of the reaction of decoupling ubiquinone with bovine mitochondrial respiratory complex I
复制标题
泛醌与牛线粒体呼吸复合物 I 解偶联反应的表征
DOI：
10.1080/09168451.2016.1179095
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
H.
中科院分区：
文献类型：
--
作者：
Masuya;T.;Okuda;K.;Murai;M. and Miyoshi;H.
我们以前生产的独特的泛醌QT（“解耦”醌），在NADH-醌氧化还原与牛心线粒体NADH-泛醌氧化还原酶（复合物I）的催化还原是完全解耦跨膜结构域的质子易位。这一特征与典型的短链醌如泛醌-1的特征明显不同。为了进一步表征与复合物I的QT反应的特征，我们在此合成了三个QT类似物，QT 2-QT 4，并表征了它们的电子转移反应。我们发现，电子转移的所有方面（例如电子接受活性和膜电位形成）在这些类似物之间变化显着。QT 2作为去偶醌的特征略优于原始QT的那些上级。基于这些结果，我们得出结论，醌结合腔内的量子点的结合位置的变化依赖于其侧链结构，和位置，反过来，控制量子点作为电子受体的行为。
We previously produced the unique ubiquinone QT (“decoupling” quinone), the catalytic reduction of which in NADH-quinone oxidoreduction with bovine heart mitochondrial NADH-ubiquinone oxidoreductase (complex I) is completely decoupled from proton translocation across the membrane domain. This feature is markedly distinct from those of typical short-chain quinones such as ubiquinone-1. To further characterize the features of the QT reaction with complex I, we herein synthesized three QT analogs, QT2–QT4, and characterized their electron transfer reactions. We found that all aspects of electron transfer (e.g. electron-accepting activity and membrane potential formation) vary significantly among these analogs. The features of QT2 as decoupling quinone were slightly superior to those of original QT. Based on these results, we conclude that the bound positions of QTs within the quinone binding cavity susceptibly change depending on their side-chain structures, and the positions, in turn, govern the behavior of QTs as electron acceptors.