The final fates of neurogenin2-expressing cells include all major neuron types in the mouse retina
The final fates of neurogenin2-expressing cells include all major neuron types in the mouse retina
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DOI：
10.1016/j.mcn.2005.10.018
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Wang, SZ
中科院分区：
文献类型：
--
作者：
Ma, WX;Wang, SZ
脊椎动物视网膜发育的分子机制还不是很清楚。为了研究神经生成素2(Ngn2)的表达是否决定了视网膜中细胞的命运，我们使用条件二进制Creer(TM)-LacZ系统绘制了曾经表达Ngn2的细胞的最终命运。我们在小鼠视网膜的所有3个核层都发现了LacZ(+)细胞，包括所有主要类型的神经元：光感受器细胞、水平细胞、双极细胞、无长突细胞和神经节细胞。LacZ(+)细胞在3个核层中的分布与视网膜细胞总数的理论分布非常相似。标记每种细胞类型的时间窗口看起来不是随机的，与其出生日期和出生顺序相似。这些数据表明，表达Ngn2的细胞在其生活史的某个时间点可能会在以后分化为各种类型的细胞。(C)2005 Elsevier Inc.保留所有权利。
The molecular mechanism underlying vertebrate retinal development is not well understood. To examine whether neurogenin2 (ngn2) expression determines cell fate in the retina, we mapped the final fates of cells that once expressed ngn2, using the conditional, binary CreER (TM)-LacZ system. We found LacZ(+) cells in all 3 nuclear layers of the mouse retina and including all major types of neurons: photoreceptors, horizontal, bipolar, amacrine, and ganglion cells. The distribution of LacZ(+) cells among the 3 nuclear layers closely resembled a theoretical distribution of total retinal cells. The temporal window in which each cell type was marked appeared nonrandom, and was similar to its birthdate and birth sequence. These data indicate that cells expressing ngn2 at some point in their life histories may later differentiate into a variety of cell types. (c) 2005 Elsevier Inc. All rights reserved.