Inhibitory effect of green tea in the drinking water on tumorigenesis by ultraviolet light and 12-O-tetradecanoylphorbol-13-acetate in the skin of SKH-1 mice.
Inhibitory effect of green tea in the drinking water on tumorigenesis by ultraviolet light and 12-O-tetradecanoylphorbol-13-acetate in the skin of SKH-1 mice.
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发表时间：
1992-03
期刊：
影响因子：
11.2
通讯作者：
Z. Y. Wang;Mou-tuan Huang;Thomas Ferraro;Ching-Quo Wong;Y. Lou;Kenneth R. Reuhl;Michael J. Iatropoulos;Chung S. Yang;Allan H. Conney
中科院分区：
文献类型：
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作者：
Z. Y. Wang;Mou-tuan Huang;Thomas Ferraro;Ching-Quo Wong;Y. Lou;Kenneth R. Reuhl;Michael J. Iatropoulos;Chung S. Yang;Allan H. Conney
以12.5 g绿茶为原料，用500 ml沸水提取2次，并将其混合制成绿茶。这种1.25%的绿茶提取物（1.25克茶叶/100毫升水）每毫升含有4.69毫克绿茶提取物固体，其成分与人类饮用的一些绿茶饮料相似。2.5%的绿茶提取物（2.5克茶叶/100毫升水）也用同样的方法制备。用180 mJ/cm2的紫外线B （UVB）照射雌性SKH-1小鼠，每天1次，连续7天，导致皮肤出现红色晒伤。在UVB治疗前和治疗期间，用1.25或2.5%的绿茶提取物作为唯一的饮用水，以剂量依赖性的方式抑制了这些病变的红色强度和面积。雌性SKH-1小鼠每天一次180mj /cm2的UVB治疗10天，1周后每周两次应用12- o -十四烷醇-13-醋酸酯治疗25周，导致皮肤肿瘤的发展。在UVB治疗前和10天期间以及UVB治疗后1周，以1.25%绿茶提取物作为唯一的饮用水，可以抑制皮肤肿瘤的形成。在另外的实验中，雌性SKH-1小鼠被200 nmol的7,12-二甲基苯(a)蒽处理，然后在3周后以180、60或30 mJ/cm2的UVB照射，每周两次，持续30周。在UVB治疗30周之前和期间，用1.25%绿茶提取物作为唯一的饮用水，可以抑制UVB诱导的皮肤肿瘤的形成和脾脏的增大。在这些实验中，用绿茶提取物治疗的动物不仅减少了皮肤肿瘤的数量，而且大大缩小了肿瘤的大小。在另外的研究中，SKH-1小鼠通过局部应用200 nmol的7,12-二甲基苯(a)蒽引发，然后每周两次应用12- o -十四烷基酚-13-醋酸酯，持续25周。在12- o -十四烷酚-13-醋酸酯的推广过程中，将1.25%的绿茶提取物作为唯一的饮用水来源，减少了皮肤肿瘤的数量和发病率。
Green tea was prepared by extracting 12.5 g of green tea leaves twice with 500 ml of boiling water, and the extracts were combined. This 1.25% green tea extract (1.25 g of tea leaves/100 ml of water) contained 4.69 mg of green tea extract solids per ml and was similar in composition to some green tea beverages consumed by humans. A 2.5% green tea extract (2.5 g of tea leaves/100 ml of water) was prepared similarly. Treatment of female SKH-1 mice with 180 mJ/cm2 of ultraviolet B light (UVB) once daily for 7 days resulted in red sunburn lesions of the skin. The intensity of red color and area of these lesions were inhibited in a dose-dependent fashion by the administration of 1.25 or 2.5% green tea extract as the sole source of drinking water before and during UVB treatment. Treatment of female SKH-1 mice with 180 mJ/cm2 of UVB once daily for 10 days followed 1 wk later by twice weekly application of 12-O-tetradecanoylphorbol-13-acetate for 25 wk resulted in the development of skin tumors. The formation of skin tumors was inhibited by administration of 1.25% green tea extract as the sole source of drinking water prior to and during the 10 days of UVB treatment and for 1 wk after UVB treatment. In additional experiments, female SKH-1 mice were treated with 200 nmol of 7,12-dimethylbenz(a)anthracene followed 3 wk later by irradiation with 180, 60, or 30 mJ/cm2 of UVB twice weekly for 30 wk. UVB-induced formation of skin tumors and increased spleen size were inhibited by administration of 1.25% green tea extract as the sole source of drinking water prior to and during the 30 wk of UVB treatment. In these experiments, treatment of the animals with the green tea extract not only decreased the number of skin tumors but also decreased substantially the size of the tumors. In additional studies, SKH-1 mice were initiated by topical application of 200 nmol of 7,12-dimethylbenz(a)anthracene followed by twice weekly application of 12-O-tetradecanoylphorbol-13-acetate for 25 wk. Administration of 1.25% green tea extract as the sole source of drinking water during promotion with 12-O-tetradecanoylphorbol-13-acetate reduced the number and incidence of skin tumors.