Post-traumatic moderate systemic hypothermia reduces TUNEL positive cells following spinal cord injury in rat
Post-traumatic moderate systemic hypothermia reduces TUNEL positive cells following spinal cord injury in rat
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DOI：
10.1038/sj.sc.3101516
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发表时间：
2004
期刊：
影响因子：
2.2
通讯作者：
S. Shibuya;O. Miyamoto;N. Janjua;T. Itano;S. Mori;H. Norimatsu
中科院分区：
文献类型：
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作者：
S. Shibuya;O. Miyamoto;N. Janjua;T. Itano;S. Mori;H. Norimatsu
研究设计：采用标准化的脊髓挫伤伴不完全性截瘫动物模型，验证中度全身低温可减少神经细胞死亡的假说。目的：探讨亚低温对大鼠脊髓损伤后神经细胞死亡的保护作用。地点：日本香川医科大学。方法：雄性SD大鼠39只，平均体重300g(280~320g)，制作脊髓损伤模型。分别于伤后24 h、72 h、7 d处死大鼠。脊髓整块切除，分5个节段进行检查：损伤中心吻侧5 mm和10 mm，损伤中心5 mm和10 mm，损伤中心尾侧5 mm和10 mm。低温组(32C/4h)与常温组(37C/3h)在同一时间点处死。苏木精-伊红染色，原位缺口末端标记(TUNEL)，一种显示脊髓细胞死亡的特异性方法。结果：与常温组相比，低温动物伤后24小时，损伤中心10 mm处TUNEL阳性细胞(TPC)明显减少。在脊髓损伤后72小时，与常温动物相比，损伤中心吻侧5 mm处以及损伤中心尾侧5 mm和10 mm处的TPC也显著降低。伤后7天，与常温动物相比，脊髓损伤后5 mm处和5 mm处的TPC均显著降低。结论：全身低温通过减轻创伤后TPC对脊髓损伤具有神经保护作用。
Study design: A standardized animal model of contusive spinal cord injury (SCI) with incomplete paraplegia was used to test the hypothesis that moderate systemic hypothermia reduces neural cell death. Terminal deoxynucleotidyl transferase [TdT]-mediated deoxyuridine triphosphate [dUTP] nick-end labeling (TUNEL) staining was used as a marker of apoptosis or cell damage.Objective: To determine whether or not moderate hypothermia could have a neuroprotective effect in neural cell death following spinal cord injury in rats.Setting: Kagawa Medical University, Japan.Methods: Male Sprague–Dawley (SD) rats (n= 39) weighing on average 300 g (280–320 g) were used to prepare SCI models. After receiving contusive injury at T11/12, rats were killed at 24 h, 72 h, or 7 days after injury. The spinal cord was removed en bloc and of examined at five segments: 5 and 10 mm rostral to the center of injury, center of injury, and 5 and 10 mm caudal to the center of injury. Rats that received hypothermia (32 C/4 h) were killed at the same time points as those that received normothermia (37 C/3 h). The specimens were stained with hematoxylin and eosin, and subjected to in situ nick-end labeling (TUNEL), a specific method for visualizing cell death in the spinal cord.Results: At 24 h postinjury, TUNEL positive cells (TPC) decreased significantly 10 mm rostral to center of injury in hypothermic animals compared to the normothermia group. At 72 h post-SCI, TPC also decreased significantly at 5 mm rostral, and 5 and 10 mm caudal to the lesion center compared to normothermic animals. At 7 days postinjury, a significant decrease of TPC was observed at the 5 mm rostral and 5 mm caudal sites compared to normothermic animals.Conclusion: These results indicate that systemic hypothermia has a neuroprotective effect following SCI by attenuating post-traumatic TPC.