Orientational order of Australian spider silks as determined by solid-state NMR
Orientational order of Australian spider silks as determined by solid-state NMR
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DOI：
10.1002/bip.20471
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发表时间：
2006-06-05
期刊：
影响因子：
2.9
通讯作者：
Separovic, F
中科院分区：
文献类型：
--
作者：
Bonev, B;Grieve, S;Separovic, F
用固体核磁共振方法研究了两种澳大利亚圆网蜘蛛（Nephila edulis和Argiope keyserlingi）的富含C-13和N-15的丝的结构。碳-13和N-15光谱从丙氨酸或甘氨酸标记的取向拖丝获得与纤维轴对齐平行或垂直于磁场。取向组分的分数由每个氨基酸、丙氨酸和甘氨酸确定，独立地使用每个核，并归因于丝中的有序结晶域。有序丙氨酸的相对分数被认为是高于有序甘氨酸的分数。类似于在蚕（家蚕）丝中观察到富含丙氨酸的区域。在N. edulis与A. Keyserlingi，这与前者的上级机械性能相关。(c)2006 Wiley Periodicals，Inc.
A simple solid-state NMR method was used to study the structure of C-13- and N-15-enriched silk from two Australian orb-web spider species, Nephila edulis and Argiope keyserlingi. Carbon-13 and N-15 spectra from alanine- or glycine-labeled oriented dragline silks were acquired with the fiber axis aligned parallel or perpendicular to the magnetic field. The fraction of oriented component was determined from each amino acid, alanine and glycine, using each nucleus independently, and attributed to the ordered crystalline domains in the silk. The relative fraction of ordered alanine was found to be higher than the fraction of ordered glycine. akin to tire observation of alanine-rich domains in silk-worm (Bombyx mori) silk. A higher degree of crystallinity was observed in the dragline silk of N. edulis compared with A. keyserlingi, which correlates with the superior mechanical properties of the former. (c) 2006 Wiley Periodicals, Inc.