Determination of protein structural ensembles using cryo-electron microscopy
Determination of protein structural ensembles using cryo-electron microscopy
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DOI：
10.1016/j.sbi.2018.10.006
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发表时间：
2019-06-01
影响因子：
6.8
通讯作者：
Vendruscolo, Michele
中科院分区：
文献类型：
--
作者：
Bonomi, Massimiliano;Vendruscolo, Michele
对蛋白质的结构、热力学和动力学的了解大大促进了对蛋白质行为的全面了解。所有这些信息都可以在结构集成方面以有效的方式提供。结构系综可以通过确定蛋白质可以占据的所有主要状态的结构、群体和相互转换率来获得。为了达到这一目标，结合联合收割机实验和计算方法的综合方法提供了强大的工具。在这里，我们专注于冷冻电子显微镜，这已成为近年来的一个宝贵的资源，桥梁的差距差距从有序无序的结构生物学。在这篇综述中，我们提供了一个角度来看，目前的挑战和机遇，在确定蛋白质结构合奏使用综合的方法，可以联合收割机冷冻电子显微镜数据与其他可用的信息来源，沿着概述了社区可用的工具。
Achieving a comprehensive understanding of the behaviour of proteins is greatly facilitated by the knowledge of their structures, thermodynamics and dynamics. All this information can be provided in an effective manner in terms of structural ensembles. A structural ensemble can be obtained by determining the structures, populations and interconversion rates for all the main states that a protein can occupy. To reach this goal, integrative methods that combine experimental and computational approaches provide powerful tools. Here we focus on cryo-electron microscopy, which has become over recent years an invaluable resource to bridge the gap from order to disorder in structural biology. In this review, we provide a perspective of the current challenges and opportunities in determining protein structural ensembles using integrative approaches that can combine cryo-electron microscopy data with other available sources of information, along with an overview of the tools available to the community.