Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes.
Live Imaging of Intracellular Dynamics During Meiotic Maturation in Mouse Oocytes.
复制标题
小鼠卵母细胞减数分裂成熟过程中细胞内动态的实时成像。
DOI：
10.1007/978-1-4939-3795-0_18
复制
发表时间：
2016
影响因子：
--
通讯作者：
T. Kitajima
中科院分区：
文献类型：
--
作者：
Shuhei Yoshida;Yogo Sakakibara;T. Kitajima
荧光实时成像是研究细胞分裂过程中细胞内动力学的一种有效方法。通过显微注射引入荧光蛋白后，小鼠卵母细胞可以在体外诱导减数分裂成熟，通过对小鼠卵母细胞应用自动共聚焦实时成像，可以高分辨率地监测细胞内结构（如染色体）的减数分裂动力学。这种方法与特定蛋白质的扰动方法相结合，为理解哺乳动物减数分裂的分子和细胞内基础提供了机会。
Fluorescence live imaging is a powerful approach to study intracellular dynamics during cellular events such as cell division. By applying automated confocal live imaging to mouse oocytes, in which meiotic maturation can be induced in vitro after the introduction of fluorescent proteins through microinjection, the meiotic dynamics of intracellular structures, such as chromosomes, can be monitored at high resolution. A combination of this method with approaches for the perturbation of specific proteins opens up opportunities for understanding the molecular and intracellular basis of mammalian meiosis.
影响因子：
16.6
作者：
Sakakibara Y;Hashimoto S;Nakaoka Y;Kouznetsova A;Höög C;Kitajima TS
通讯作者：
Kitajima TS