Studies on the Regulation of Insulin Binding by Liver Plasma Membranes From Zucker Fatty Rats
Studies on the Regulation of Insulin Binding by Liver Plasma Membranes From Zucker Fatty Rats
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Zucker脂肪大鼠肝质膜对胰岛素结合调节的研究
DOI：
10.2337/diab.31.10.867
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发表时间：
1982
期刊：
影响因子：
7.7
通讯作者：
C. M. Clark
中科院分区：
文献类型：
--
作者：
J. Clark;S. Keen;C. M. Clark
除了肥胖的Zucker大鼠的肝细胞外，肥胖啮齿动物的高胰岛素血症与肝脏胰岛素受体的数量减少有关。我们分离了10-11周龄瘦小和肥胖的Zucker大鼠的肝脏质膜，其中一些大鼠在2-4周前注射了链脲佐菌素。我们已经确定，虽然幼年高胰岛素血症肥胖大鼠的肝脏胰岛素受体数量没有减少，但当用链脲佐菌素治疗脂肪大鼠的高胰岛素血症时，受体数量可以增加。在肥胖大鼠中，循环胰岛素的减少伴随着血浆甘油三酯浓度的下降，这与肝脏脂肪生成的刺激减少是一致的。用分离的肝细胞质膜和~(125)I-胰岛素测定了胰岛素的竞争结合曲线。对这些亲和力和受体数量曲线的分析表明，脂肪控制大鼠的胰岛素受体数量与瘦身对照组大鼠相比没有变化，但在链脲佐菌素处理的动物中胰岛素受体数量增加。这些数据表明，在幼年肥胖的Zucker大鼠中，肝脏胰岛素受体的调节发生了变化，其特征是高胰岛素血症使肝脏胰岛素受体缺乏下调，而当胰岛素浓度高于瘦鼠时，肝脏胰岛素受体上调。肝脏胰岛素受体调节状态的改变可能是肥胖的特征。
The hyperinsulinemia of obese rodents has been associated with a reduced number of hepatic insulin receptors except in hepatocytes from fatty Zucker rats. We isolated liver plasma membranes from 10–11-wk-old lean and fatty Zucker rats, some of which were injected with streptozotocin 2–4 wk earlier. We have determined that although the number of hepatic insulin receptors is not reduced in young hyperinsulinemic fatty Zucker rats, the number of receptors can be increased when the hyperinsulinemia of the fatty rats is reduced by treatment with streptozotocin. In the fatty rats, this reduction in circulating insulin is accompanied by a reduction in plasma triglyceride concentration, consistent with a decreased stimulation of hepatic lipogenesis. Competitive binding curves for insulin were obtained with isolated liver plasma membranes and 125I-insulin. Analysis of these curves for affinity and number of receptors indicated that the number of insulin receptors was unchanged for the fatty control rats relative to the lean control rats but was increased in streptozotocin-treated animals. These data indicate that the regulation of hepatic insulin receptors is altered in the young fatty Zucker rat as characterized by a lack of downregulation of hepatic insulin receptors by hyperinsulinemia and an upregulation of hepatic insulin receptors at insulin concentrations higher than those found in lean rats. An altered state of hepatic insulin receptor regulation may be characteristic of developing obesity.
肥胖中胰岛素受体的饮食调节。
DOI：
10.1093/jn/111.3.475
发表时间：
1981
期刊：
The Journal of nutrition
影响因子：
--
作者：
Misbin,RI
通讯作者：
Misbin,RI