DIVALENT-CATION REGULATION OF THE SURFACE ORIENTATION OF PLATELET MEMBRANE GLYCOPROTEIN LLB - CORRELATION WITH FIBRINOGEN BINDING FUNCTION AND DEFINITION OF A NOVEL VARIANT OF GLANZMANNS-THROMBASTHENIA
DIVALENT-CATION REGULATION OF THE SURFACE ORIENTATION OF PLATELET MEMBRANE GLYCOPROTEIN LLB - CORRELATION WITH FIBRINOGEN BINDING FUNCTION AND DEFINITION OF A NOVEL VARIANT OF GLANZMANNS-THROMBASTHENIA
复制标题
DOI：
10.1172/jci112667
复制
发表时间：
1986-10-01
影响因子：
15.9
通讯作者：
PLOW, EF
中科院分区：
文献类型：
--
作者：
GINSBERG, MH;LIGHTSEY, A;PLOW, EF
抗血小板单克隆抗体PMI-1与糖蛋白（GP）GPIIB，游离GPIIB和GPIIB-IIIA复合物反应。该抗体与每个血小板的40,900个位点结合，kd = 0.95 .mu.m，其结合受到悬浮培养基中镁或钙的存在抑制（.apprx。0.5mm分裂阳离子的50％抑制）。 PMI-1表位的调节独立于GPIIB-IIIA异二聚体的拆卸，因为它发生在22.级。 C和对MM镁和钙的响应。 PMI-1结合与纤维蛋白原的结合呈负相关。此外，我们确定了Glanzmann's Throlombasthenia的一种变体，其GPIIB-IIIA异二聚体的接近正常的血小板含量是通过交叉免疫电信和表面标记来判断的。 PMI-1与这些患者的血小板的结合不取决于降低二价阳离子浓度。这些数据表明，GPIIB的表面取向在血小板结合纤维蛋白原的能力中很重要。
An antiplatelet monoclonal antibody, PMI-1, reacts with glycoproteins (GP) GPIIb, free GPIIb, and the GPIIb-IIIa complex. This antibody binds to 40,900 sites per platelet, with a Kd = 0.95 .mu.M, and its binding is inhibited by the presence of magnesium or calcium in the suspending medium (50% suppression at .apprx. 0.5 mM divalent cation). Regulation of the PMI-1 epitope is independent of disassembly of the GPIIb-IIIa heterodimer, because it occurred at 22.degree. C and in response to mM magnesium as well as calcium. PMI-1 binding inversely correlated with fibrinogen binding. In addition, we identified a variant of Glanzmann''s thrombasthenia with near-normal platelet content of the GPIIb-IIIa heterodimer as judged by crosses immunoelectrophoresis and surface labeling. Binding of PMI-1 to these patients'' platelets was not dependent on reduction of the divalent cation concentration. These data suggest that the surface orientation of GPIIb is important in the capacity of platelets to bind fibrinogen.