Identification of a neutralization epitope on the envelope gp46 antigen of human T cell leukemia virus type I and induction of neutralizing antibody by peptide immunization.
Identification of a neutralization epitope on the envelope gp46 antigen of human T cell leukemia virus type I and induction of neutralizing antibody by peptide immunization.
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人 T 细胞白血病病毒 I 型包膜 gp46 抗原上中和表位的鉴定以及通过肽免疫诱导中和抗体。
DOI：
10.4049/jimmunol.147.1.354
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发表时间：
1991
影响因子：
4.4
通讯作者：
H. Tozawa
中科院分区：
文献类型：
--
作者：
Yuetsu Tanaka;L. Zeng;Hiroshi Shiraki;Hisatoshi Shida;H. Tozawa
我们已经产生了一些单克隆抗体的外部包膜糖蛋白，gp 46，人T细胞白血病病毒I型（HTLV-I）从WKA大鼠免疫含有HTLV-I env基因的重组牛痘病毒的各种表位。在这些mAb中，如通过HTLV-I介导的细胞融合抑制测定所确定的，一组mAb（以命名为LAT-27的mAb为代表）可以中和HTLV-I的感染性。LAT-27还在体外干扰HTLV-I对正常T淋巴细胞的转化。使用重叠合成寡肽的抗体结合测定显示LAT-27特异性结合包含gp 46氨基酸序列191-196（Leu-Pro-His-Ser-Asn-Leu）的10聚体肽。来自HTLV-I+人的抗体干扰LAT-27与gp 46 Ag的结合。来自用与OVA缀合的LAT-27反应性肽190-199免疫的兔的血清，而不是来自OVA免疫的兔的血清，与gp 46 Ag反应并中和HTLV-1的感染性。这些结果表明，由LAT-27识别的HTLV-I中和表位位于gp 46氨基酸191-196，并且用含有LAT-27表位的肽免疫可以引发HTLV-I中和抗体应答。
We have generated a number of mAb against various epitopes on the external envelope glycoprotein, gp46, of human T cell leukemia virus type I (HTLV-I) from a WKA rat immunized with a recombinant vaccinia virus containing the HTLV-I env gene. Among these mAb, one group of mAb, represented by a mAb designated LAT-27, could neutralize the infectivity of HTLV-I, as determined by a HTLV-I-mediated cell fusion inhibition assay. LAT-27 also interfered with transformation of normal T lymphocytes by HTLV-I in vitro. An antibody-binding assay using overlapping synthetic oligopeptides showed that LAT-27 bound specifically to 10-mer peptides that contained the gp46 amino acid sequence 191-196 (Leu-Pro-His-Ser-Asn-Leu). Antibodies from HTLV-I+ humans interfered with the binding of LAT-27 to gp46 Ag. Sera from rabbits immunized with a LAT-27-reactive peptide, 190-199, conjugated with OVA, but not sera from OVA-immunized rabbits, reacted with gp46 Ag and neutralized infectivity of HTLV-I. These results show that the HTLV-I neutralization epitope recognized by LAT-27 locates to the gp46 amino acids 191-196, and that immunization with a peptide containing the LAT-27 epitope can elicit an HTLV-I neutralizing antibody response.