Gut microbial metabolism of dietary fibre protects against high energy feeding induced ovarian follicular atresia in a pig model
Gut microbial metabolism of dietary fibre protects against high energy feeding induced ovarian follicular atresia in a pig model
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膳食纤维的肠道微生物代谢可预防猪模型中高能量喂养引起的卵巢卵泡闭锁
DOI：
10.1017/s0007114520002378
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发表时间：
2021-01-14
影响因子：
3.6
通讯作者：
Wu, De
中科院分区：
文献类型：
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作者：
Zhuo, Yong;Cao, Meng;Wu, De
摘要为了研究饲粮纤维对高脂肪饲粮猪卵泡闭锁的影响，本试验选用32头青春期前后备母猪，分别饲喂基础饲粮（CON）和基础饲粮中添加300 g/d膳食纤维（纤维）、240 g/d大豆油（SO）或两者（纤维+ SO）的饲粮。在第4个发情期第19天，饲喂粗纤维日粮的后备母猪卵泡闭锁率增加了112%，凋亡标志物Bax和caspase-3的表达增加，而饲喂纤维日粮的后备母猪的这种效应被逆转。产scfa微生物的丰度被SO日粮降低，但这种影响被纤维处理逆转。血清和卵泡液中血清素和褪黑素的浓度因纤维饮食而升高。总的来说，膳食纤维至少部分通过与肠道微生物群相关的血清素-褪黑素合成来防止高脂肪喂养引起的卵泡闭锁。这些结果为防止高能量饮食对人类生育能力的负面影响提供了见解。
Abstract To investigate the effects of dietary fibre on follicular atresia in pigs fed a high-fat diet, we fed thirty-two prepubescent gilts a basal diet (CON) or a CON diet supplemented with 300 g/d dietary fibre (fibre), 240 g/d soya oil (SO) or both (fibre + SO). At the 19th day of the 4th oestrus cycle, gilts fed the SO diet showed 112 % more atretic follicles and greater expression of the apoptotic markers, Bax and caspase-3, and these effects were reversed by the fibre diet. The abundance of SCFA-producing microbes was decreased by the SO diet, but this effect was reversed by fibre treatment. Concentrations of serotonin and melatonin in the serum and follicular fluid were increased by the fibre diet. Overall, dietary fibre protected against high fat feeding-induced follicular atresia at least partly via gut microbiota-related serotonin–melatonin synthesis. These results provide insight into preventing negative effects on fertility in humans consuming a high-energy diet.