Yellowing disease of tomato caused by Tomato chlorosis virus newly recognized in Japan
Yellowing disease of tomato caused by Tomato chlorosis virus newly recognized in Japan
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DOI：
10.1007/s10327-010-0219-4
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发表时间：
2010-04-01
影响因子：
1.2
通讯作者：
Okuda, Seiichi
中科院分区：
文献类型：
--
作者：
Hirota, Tomoki;Natsuaki, Tomohide;Okuda, Seiichi
2008年，在栃木县的温室番茄植株（Solanum esculentum）上观察到黄化、脉间失绿、叶片卷曲和坏死斑点的病毒样症状。病原体的病原学和特征如粉虱传播性和颗粒形态与梭状病毒科中的Crinivirus属的番茄传染性褪绿病毒（TICV）和番茄褪绿病毒（ToCV）的病原学和特征相似。使用线形病毒热休克蛋白70同源基因的简并引物和TICV和ToCV的特异性引物对逆转录-聚合酶链反应（RT-PCR）产物进行测序，结果表明该病毒为ToCV，此前在日本尚未报道。
In 2008, virus-like symptoms of yellowing, interveinal chlorosis, leaf curling and necrotic fleck were observed on greenhouse-tomato plants (Solanum esculentum) in Tochigi Prefecture. The symptomatology and the characteristics of the causal agent such as whitefly transmissibility and particle morphology are similar to those for Tomato infectious chlorosis virus (TICV) and Tomato chlorosis virus (ToCV), species of the genus Crinivirus in the family Closteroviridae. Sequencing of reverse transcription-polymerase chain reaction (RT-PCR) products using the degenerate primers for heat shock protein 70 homolog genes of closteroviruses and specific primers for TICV and ToCV indicated that the virus was ToCV, that has not previously been reported in Japan.