Stability of dendritic spines and synaptic contacts is controlled by alpha N-catenin.
Stability of dendritic spines and synaptic contacts is controlled by alpha N-catenin.
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发表时间：
2004
影响因子：
25
通讯作者：
K. Abe;O. Chisaka;F. van Roy;M. Takeichi
中科院分区：
文献类型：
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作者：
K. Abe;O. Chisaka;F. van Roy;M. Takeichi
树突棘和突触的形态可塑性被认为对它们的生理功能至关重要。本研究表明，钙粘蛋白粘附受体和肌动蛋白细胞骨架之间的连接物α - n -连环蛋白对于稳定培养的啮齿动物海马神经元的树突棘至关重要。在缺乏α - n -连环蛋白的情况下，棘头异常运动，主动从突触接触部位伸出丝状足。相反，α n -连环蛋白在树突中的过度表达减少了脊柱的更新，导致脊柱和突触密度的增加。河豚毒素（TTX）是一种神经活动阻滞剂，抑制α - n -连环蛋白的突触积累，而GABA拮抗剂双丘碱则促进α - n -连环蛋白的突触积累。此外，过量的- n -连环蛋白使脊柱对TTX治疗产生抗性。这些结果表明- n -连环蛋白是突触接触稳定性的关键调节因子。
Morphological plasticity of dendritic spines and synapses is thought to be crucial for their physiological functions. Here we show that alpha N-catenin, a linker between cadherin adhesion receptors and the actin cytoskeleton, is essential for stabilizing dendritic spines in rodent hippocampal neurons in culture. In the absence of alpha N-catenin, spine heads were abnormally motile, actively protruding filopodia from their synaptic contact sites. Conversely, alpha N-catenin overexpression in dendrites reduced spine turnover, causing an increase in spine and synapse density. Tetrodotoxin (TTX), a neural activity blocker, suppressed the synaptic accumulation of alpha N-catenin, whereas bicuculline, a GABA antagonist, promoted it. Furthermore, excess alpha N-catenin rendered spines resistant to the TTX treatment. These results suggest that alpha N-catenin is a key regulator for the stability of synaptic contacts.