cDNA cloning and phylogenetic and expression analyses of actin in symbiotic dinoflagellates (Symbiodinium spp.)
cDNA cloning and phylogenetic and expression analyses of actin in symbiotic dinoflagellates (Symbiodinium spp.)
复制标题
DOI：
10.1007/s10811-006-9106-y
复制
发表时间：
2006-04-01
影响因子：
3.3
通讯作者：
Maruyama, Tadashi
中科院分区：
文献类型：
--
作者：
Watanabe, Toshiki;Kii, Shin-ichi;Maruyama, Tadashi
三个编码肌动蛋白的cDNA被确定在两个可培养的菌株（分支A和F）的共生甲藻Symbiodinium spp。在分子系统发育分析中，这些肌动蛋白序列与已知的甲藻肌动蛋白形成了一个单系群，远离从进化枝A共生藻菌株（HG 39）中分离的Syact-p。其中一个新鉴定的肌动蛋白序列（SyAct-F1）与从两种礁珊瑚（Galaxea fascicularis和Favites chinensis）的成年群体中分离的部分肌动蛋白cDNA序列（命名为AGfact-p和AFcact-p）最接近，这表明后两者可能来自共生体。从共生的F. chinensis为模板，而不是来自浮游幼虫，在浮游幼虫中没有检测到虫黄藻，这也为AFcact-p起源于共生体提供了证据。表达分析表明，SyAct-A1 mRNA的水平是相当的共生和非共生状态，也在运动和非运动阶段的培养条件下，这表明其作为一个组成型表达的控制基因在共生藻mRNA的表达分析的有用性。
Three cDNAs encoding actins were identified in two culturable strains (clades A and F) of the symbiotic dinoflagellates Symbiodinium spp. In a molecular phylogenetic analysis these actin sequences formed a monophyletic group with known dinoflagellate actins, remote from Syact-p that had been isolated from a clade A Symbiodinium strain (HG39). One of the newly identified actin sequences (SyAct-F1) was the most closely related to partial actin cDNA sequences (named AGfact-p and AFcact-p) isolated from adult colonies of two reef corals (Galaxea fascicularis and Favites chinensis) that were inhabited by Symbiodinium spp., suggesting the possibility that the latter two were from the symbionts. Partial AFcact-p sequences could be amplified by PCR using genomic DNA prepared from a symbiotic adult colony of F. chinensis as the template, but not from planula larvae in which zooxanthellae could not be detected, also arguing for the origin of AFcact-p in the symbiont. An expression analysis showed that the levels of the SyAct-A1 mRNA were comparable in symbiotic and non-symbiotic states, and also in motile and non-motile phases in a cultured condition, suggesting its usefulness as a constitutively expressed control gene in expression analysis of Symbiodinium mRNAs.