Evaluation of cross-linked chitosan microparticles for bone regeneration
Evaluation of cross-linked chitosan microparticles for bone regeneration
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DOI：
10.1002/term.270
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发表时间：
2010-10-01
影响因子：
3.3
通讯作者：
Jayasuriya, A. Champa
中科院分区：
文献类型：
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作者：
Bhat, Archana;Dreifke, Michael B.;Jayasuriya, A. Champa
本初步研究的目的是评价壳聚糖（CS）基微颗粒（MPs）在体内骨再生中的应用。CS MPs是用我们的放大方法制造的，如前所述。从大鼠股骨和胫骨中获取间充质干细胞（MSC），并将其移植到CS MPs上。分别在5、10、20和30 h对粘附在MPs上的细胞进行胰蛋白酶化，并在24和48 h采用活/死细胞法对粘附在MPs上的MSCs进行荧光成像。将MSC/成骨细胞（OB）接种于MPs上，利用成骨培养基进行体外培养，并植入大鼠股骨部分厚度骨缺损。大鼠分为实验动物和对照两组进行体内研究。实验组分别植入msc种子MPs，于第4周和第8周进行观察。对照组大鼠除用不锈钢板支撑缺损外，不接受任何种植材料。每组4只大鼠。在植入后4周和8周取出股骨，利用x线摄影、显微计算机断层扫描（mu CT）和组织学分析缺损部位的骨形成情况。在所有组中，实验组在种植8周时骨形成明显增加。本研究结果表明，CS MPs被证明是一种成功的骨再生生物材料。版权所有John Wiley & Sons， Ltd。
The objective of this preliminary study was to evaluate the applying of chitosan (CS)-based microparticles (MPs) in bone regeneration in vivo. The CS MPs were fabricated using our scale-up method, as previously described. Mesenchymal stem cells (MSC) were harvested from the femora and tibiae of Dark Agouti (DA) rats and seeded on CS MPs. An in vitro MSCs attachment experiment was conducted by trypsinizing the cells attached to the MPs at 5, 10, 20 and 30 h. Fluorescence images of MSCs attached to the MPs were taken at 24 and 48 h, using a LIVE/DEAD cell assay. The MSC/osteoblasts (OB) seeded on MPs were then cultured in vitro using osteogenic media and implanted into partial thickness bone defects in rat femurs. There were two groups of rats, including experimental animals and controls, for the in vivo studies. The experimental group were implanted with MSC-seeded MPs and observed at 4 and 8 weeks. The control group of rats did not receive any implant material except the stainless steel plate to support the defect. Four rats per group were used for the study. The femurs were extracted at 4 and 8 weeks post-implantation and bone formation at the defect site was analysed using radiography, microcomputed tomography (mu CT) and histology. Among all groups, a significant increase in bone formation was observed in the experimental group at 8 weeks implantation. The results of this study suggested that CS MPs prove to be a successful biomaterial for bone regeneration. Copyright (C) 2010 John Wiley & Sons, Ltd.