RED-CELL MEMBRANE GLYCOPHORIN LABELING FROM WITHIN LIPID BILAYER
RED-CELL MEMBRANE GLYCOPHORIN LABELING FROM WITHIN LIPID BILAYER
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DOI：
10.1126/science.663661
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发表时间：
1978-01-01
期刊：
影响因子：
56.9
通讯作者：
GITLER, C
中科院分区：
文献类型：
--
作者：
KAHANE, I;GITLER, C
Human red blood cell membranes were labeled from within the lipid bilayer by the apolar photosensitive reagent, 5-[125I]iodonaphthyl-1-azide. Glycophorin, the major sialoglycoprotein of the red cell membrane, was purified by 2 different methods. It contained approximately half of the total label incorporated into membrane proteins. The label was confined to the trypsin-insoluble peptide of glycophorin that includes a sequence of 20, mainly apolar, amino acids. The labeled segment probably resides within the membrane in direct contact with the lipid bilayer. The suggestion that glycophorin spans the bilayer through its hydrophobic domain was supported.
