HuD Regulates the cpg15 Expression Via the 3′-UTR and AU-Rich Element
HuD Regulates the cpg15 Expression Via the 3′-UTR and AU-Rich Element
复制标题
DOI：
10.1007/s11064-011-0443-0
复制
发表时间：
2011-03
影响因子：
4.4
通讯作者：
Zhong-Hui Wang;Shu-Jing Li;Y. Qi;Jing-jing Zhao;Xiao-Yan Liu;Yu Han;P. Xu;Xian-Hua Chen
中科院分区：
文献类型：
--
作者：
Zhong-Hui Wang;Shu-Jing Li;Y. Qi;Jing-jing Zhao;Xiao-Yan Liu;Yu Han;P. Xu;Xian-Hua Chen
候选可塑性相关基因15（cpg15）在神经发育和可塑性中起重要作用。本研究探讨了cpg15 3 ′-非翻译区（UTR）在调控该基因表达中的作用。结果表明，cpg15基因3 ′-UTR的表达显著降低，而3 ′-UTR中富含AU的元件（ARE）的缺失使cpg15的表达增加，表明3 ′-UTR和ARE可能是调节cpg15表达的关键。此外，神经特异性RNA结合蛋白HuD可增加cpg15的表达，这依赖于3 ′-UTR和ARE的存在。RNA结合蛋白免疫沉淀（RIP）实验表明，HuD与cpg15 mRNA在大鼠海马细胞中形成复合物。HuD结构域RRM1加RRM2或铰链区加RRM3的缺失减弱了HuD在增强cpg15表达中的功能。结果表明，HuD通过3 ′-UTR介导的机制调控cpg15的表达，该机制需要ARE的存在。
The candidate plasticity related gene 15 (cpg15) plays important roles in neural development and plasticity. In the present study, we studied the role of the cpg15 3′-untranslated region (UTR) in regulating the expression of the gene. The results showed that the presence of the 3′-UTR significantly decreases, while loss of a putative AU-rich element (ARE) in the 3′-UTR increases the cpg15 expression, indicating that the 3′-UTR and ARE may be essential for regulation of cpg15 expression. In addition, HuD, a neural-specific RNA binding protein, increased the cpg15 expression, which depends on the presence of the 3′-UTR and ARE. RNA-binding protein immunoprecipitation (RIP) assay demonstrated that HuD forms a complex with cpg15 mRNA in the cells of rat hippocampus. Deletion of HuD domains RRM1 plus RRM2 or Hinge region plus RRM3 attenuates the function of HuD in enhancing the cpg15 expression. The results suggest that HuD regulates the cpg15 expression via the 3′-UTR-mediated mechanism, which requires the presence of the ARE.