HEPATITIS-B VIRUS GENE-FUNCTION - THE PRECORE REGION TARGETS THE CORE ANTIGEN TO CELLULAR MEMBRANES AND CAUSES THE SECRETION OF THE E-ANTIGEN
HEPATITIS-B VIRUS GENE-FUNCTION - THE PRECORE REGION TARGETS THE CORE ANTIGEN TO CELLULAR MEMBRANES AND CAUSES THE SECRETION OF THE E-ANTIGEN
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DOI：
10.1073/pnas.83.6.1578
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发表时间：
1986-03-01
影响因子：
11.1
通讯作者：
RUTTER, WJ
中科院分区：
文献类型：
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作者：
OU, JH;LAUB, O;RUTTER, WJ
乙肝病毒基因组的核心基因包含两个保守的同相起始密码子，间隔约90个核苷酸。这一区域(“前核心区”)编码大量疏水性氨基酸。我们利用猴病毒40衍生载体在异种哺乳动物细胞中表达了含有或不含有前C区的核心基因的编码序列。结果表明，前C区既不是表达核心抗原(CAg)所必需的，也不是表达与之相关的乙肝病毒e抗原(EAG)所必需的。然而，前核心区导致CAG与细胞膜，可能是内质网联系在一起。此外，前核序列的存在导致了EAG的分泌。我们的结果表明，前C区起到了将核心蛋白靶向到膜上的作用，这可能是EAGE分泌的直接原因，也可能有助于核心和表面抗原在病毒颗粒形成中的相互作用。
The core gene of the hepatitis B virus genome contains two conserved in-phase initiation codons separated by about 90 nucleotides. This region ("the precore region") encodes largely hydrophobic amino acids. We have expressed the coding sequence of the core gene with or without the precore region by using a simian virus 40-derived vector in heterologous mammalian cells. The results show that the precore region is not required for the expression either of core antigen (cAg) or of a related hepatitis B virus antigen, the e antigen (eAg). However, the precore region causes the cAg to become associated with cytoplasmic membranes, probably the endoplasmic reticulum. Further, the presence of the precore sequence results in the secretion of eAg. Our results suggest that the precore region plays a role in targeting core proteins to the membrane; this may be the direct cause of eAge secretion and also may aid in the interaction of the core and surface antigens in the formation of the viral particle.