Age- and gender-related oxidative status determined in healthy subjects by means of OXY-SCORE, a potential new comprehensive index
Age- and gender-related oxidative status determined in healthy subjects by means of OXY-SCORE, a potential new comprehensive index
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DOI：
10.1080/13547500600898623
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发表时间：
2006-11-01
期刊：
影响因子：
2.6
通讯作者：
Cavalca, V.
中科院分区：
文献类型：
--
作者：
Veglia, F.;Cighetti, G.;Cavalca, V.
氧化应激与各种疾病、性别和衰老有关，并通过各种标志物进行测量。作者开发了一种程序来计算整体氧化应激指数（OXY SCORE），反映健康受试者的氧化和抗氧化标志物。其性能与年龄、性别和冠状动脉疾病（CAD）患者的相关性进行了测试。82例健康受试者和20例CAD患者入组。血浆游离和总丙二醛（F-和TMDA），谷胱甘肽二硫化物/还原型比值（GSSG/GSH）和尿异前列腺素（iPF（2 α）-III）水平结合作为氧化损伤标志物（损伤评分）。GSH，α-和γ-生育酚（TH）水平，和个人的抗氧化能力相结合的抗氧化防御指数（保护得分）。通过从损伤评分中减去保护评分来计算氧合评分。在单个参数中，TMDA和iPF（2 α）-III与年龄显著相关;在健康受试者中，只有GSH和两种生育酚与男性性别相关。OXY评分与年龄（p = 0.004）和男性（p = 0.03）呈正相关。正如预期的那样，在调整年龄、性别和吸烟后，CAD患者的OXY评分较高，p值非常显著（< 0.0001）。结合不同的标志物可以潜在地提供一个强大的指标，在与年龄，性别和CAD状态相关的氧化应激的评价。
Oxidative stress has been related to various diseases, gender and ageing, and has been measured by various markers. The authors developed a procedure to compute a global oxidative stress index (OXY SCORE), reflecting both oxidative and antioxidant markers in healthy subjects. Its performance was tested in relation to age and gender and in coronary artery disease (CAD) patients. Eighty-two healthy subjects and 20 CAD patients were enrolled. Plasma free and total malondialdehyde (F- and TMDA), glutathione disulphide/reduced form ratio (GSSG/GSH) and urine isoprostanes (iPF(2 alpha)-III) levels were combined as oxidative damage markers (damage score). GSH, alpha- and gamma-tocopherol (TH) levels, and individual antioxidant capacity were combined as antioxidant defence indexes (protection score). The OXY SCORE was computed by subtracting the protection score from the damage score. Among single parameters, TMDA and iPF(2 alpha)-III significantly correlated with age; only GSH and both tocopherols correlated with male gender in healthy subjects. The OXY SCORE was positively associated with age (p = 0.004) and male gender (p = 0.03). As expected, the OXY SCORE was higher in CAD with a very significant p-value ( < 0.0001), after adjusting for age, gender and smoking. Combining different markers can potentially provide a powerful index in the evaluation of oxidative stress related to age, gender and CAD status.