Rapid hydrogel formation via tandem visible light photouncaging and bioorthogonal ligation
Rapid hydrogel formation via tandem visible light photouncaging and bioorthogonal ligation
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DOI：
10.1016/j.xcrp.2022.101185
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发表时间：
2022-12-21
影响因子：
8.9
通讯作者：
Page, Zachariah A.
中科院分区：
文献类型：
--
作者：
Chung, Kun-You;Halwachs, Kathleen N.;Page, Zachariah A.
描述了使用绿色光反应光笼在水中形成良性聚合物支架。这些努力为合成支架的制造铺平了道路，这些支架可以促进疾病诊断和治疗的细胞事件的研究。首先，检查了一系列具有含氮亲核试剂的硼二吡咯亚甲基（BODIPY）光笼以确定结构-反应性关系，其导致解除笼化产率增加> 1，0003。随后，在90wt%水中通过双正交羰基缩合使用分别含有BODIPY光笼和邻苯二甲醛（OPA）部分的亲水性聚合物支架完成光诱导水凝胶形成。时空控制证明与光开/关实验，以调节凝胶刚度和掩蔽，以提供
The formation of benign polymer scaffolds in water using green -light-reactive photocages is described. These efforts pave an avenue toward the fabrication of synthetic scaffolds that can facili-tate the study of cellular events for disease diagnosis and treatment. First, a series of boron dipyrromethene (BODIPY) photocages with nitrogen-containing nucleophiles were examined to determine structure-reactivity relationships, which resulted in a >1,0003 increase in uncaging yield. Subsequently, photoinduced hydrogel formation in 90 wt % water was accomplished via biorthogonal carbonyl condensation using hydrophilic polymer scaffolds sepa-rately containing BODIPY photocages and ortho-phthalaldehyde (OPA) moieties. Spatiotemporal control is demonstrated with light on/off experiments to modulate gel stiffness and masking to pro-vide