Use of binary solvent systems in the MALDI-TOF analysis of poly(methyl methacrylate).
Use of binary solvent systems in the MALDI-TOF analysis of poly(methyl methacrylate).
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二元溶剂系统在聚甲基丙烯酸甲酯的 MALDI-TOF 分析中的使用。
DOI：
10.1021/ac970409f
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发表时间：
1997
影响因子：
7.4
通讯作者：
Guo,B
中科院分区：
文献类型：
--
作者：
Chen,H;Guo,B
与聚合物的基质辅助激光解吸电离飞行时间（MALDI-TOF）分析相关的两个主要问题是质量歧视和再现性差。本文表明，二元溶剂系统的使用是这些问题的原因之一。据观察，在PMMA溶液中的第二溶剂，如水的存在下，可能会导致显着的质量歧视和变化的聚合物分布，这可以改变超过50%，当PMMA 4K被用作聚合物样品。一般而言，少量的第二溶剂倾向于导致不太严重的质量歧视，但变化的分布，而大量的第二溶剂更可能导致更系统和严重的质量歧视。除少数情况外，观察到质量歧视是针对较大的低聚物。此外，据观察，这种效果随基质，主要溶剂，和样品制备使用。这项工作的重要性在于，它提供了一个更好的样品制备方案，以尽量减少质量歧视和重现性差的问题的指导。查明了导致水污染的几个潜在来源。最后，一个简单的方法来验证第二种溶剂的量是否足够高，导致质量歧视也进行了讨论。
Two of the main problems associated with matrix-assisted laser desorption−ionization time-of-flight (MALDI-TOF) analysis of polymers are mass discrimination and poor reproducibility. This paper shows that the use of binary solvent systems is one of the causes of these problems. It was observed that the presence of a second solvent such as water in the PMMA solution could cause significant mass discrimination and varying polymer distributions, which can be varied by over 50% when PMMA 4K was used as the polymer sample. In general, a small amount of a second solvent tends to result in less severe mass discrimination but varying distributions, while large amounts of a second solvent are more likely to cause more systematic and severe mass discrimination. Except for a few cases, mass discrimination was observed to be against larger oligomers. Moreover, it was observed that this effect varies with the matrix, main solvent, and sample preparation used. The importance of this work is that it provides guidance for one to develop a better sample preparation protocol to minimize the mass discrimination and poor reproducibility problems. Several potential sources leading to water contamination were identified. Finally, a simple method to verify if amounts of a second solvent are sufficiently high to cause mass discrimination is also discussed.