Reinvestigation of Classic T Cell Subsets and Identification of Novel Cell Subpopulations by Single-Cell RNA Sequencing
Reinvestigation of Classic T Cell Subsets and Identification of Novel Cell Subpopulations by Single-Cell RNA Sequencing
复制标题
通过单细胞 RNA 测序重新研究经典 T 细胞亚群并鉴定新细胞亚群。
DOI：
10.4049/jimmunol.2100581
复制
发表时间：
2022-01-15
影响因子：
4.4
通讯作者：
Jin, Wenfei
中科院分区：
文献类型：
--
作者：
Wang, Xuefei;Shen, Xiangru;Jin, Wenfei
经典的T细胞亚群是由一小组细胞表面标记定义的，而单细胞RNA测序(scRNA-seq)使用基因组范围的基因表达谱对细胞进行分类。将scRNA-seq聚集群(ScCPop)与其他T细胞亚群的细胞之间的关系分配给多个scCPop，包括粘膜相关的不变T细胞和NKT细胞。形成一个scCPop的多个T细胞亚群表现出相似的表达模式，但反之亦然，这表明scCPop是BST2的FACS用于scRNA-seq分析的人外周血单核细胞。去除ISAGs后，ISAGhi T细胞群在t分布随机邻域嵌入图上消失，而ISAGhi T细胞群在ISAGs分析中出现。The Journal of the Journal of
Classic T cell subsets are defined by a small set of cell surface markers, while single-cell RNA sequencing (scRNA-seq) clusters cells using genome-wide gene expression profiles. The relationship between scRNA-seq clustered populations (scCPops) and cell from the other T cell subsets were assigned to multiple scCPops, including mucosal-associated invariant T cells and NKT cells. The multiple T cell subsets forming one scCPop exhibit similar expression patterns, but not vice versa, indicating scCPop is a from human PBMCs by FACS of BST2 for scRNA-seq analyses. The ISAGhi T cell cluster disappeared on t-distributed stochastic neighbor embedding plot after removing ISAGs, whereas the ISAGhi T cell cluster showed up by analysis of ISAGs efficiencies of T cell activation indicate that a high level of ISAGs may contribute to quick immune responses. The Journal of