Characterization of a promonocyte clone chronically infected with HIV and inducible by 13-phorbol-12-myristate acetate.
Characterization of a promonocyte clone chronically infected with HIV and inducible by 13-phorbol-12-myristate acetate.
复制标题
慢性感染 HIV 且可由 13-佛波醇-12-肉豆蔻酸酯醋酸酯诱导的早单核细胞克隆的表征。
作者：
T. Folks;J. Justement;A. Kinter;S. Schnittman;J. Orenstein;G. Poli;A. Fauci
本研究调查了 PMA（一种单核细胞分化诱导剂）对慢性感染的幼单核细胞克隆中 HIV 表达的影响。 U937细胞急性HIV感染后，通过有限稀释分离出组成型表达不同水平HIV的克隆。一个克隆 (U1) 产生的 HIV 水平较低，但通过逆转录酶或 A 捕获检测发现，在 PMA 诱导后其产量增加了 20 倍。 U1 的进一步表征表明，PMA 可以诱导克隆中的细胞分化和成熟，类似于未感染的 U937 细胞。此外，功能研究表明，U1 克隆产生的超氧阴离子与未感染的 U937 细胞没有什么不同。 U1 的电子显微镜研究表明，PMA 诱导含有许多 HIV 颗粒的内吞囊泡。这些研究提供了克隆水平的模型，以 1) 检查单核细胞中 HIV 感染的潜伏期或慢性性，2) 描绘转化为高水平病毒表达所需的信号。
The present study has investigated the effect of PMA, an inducer of monocyte differentiation, on HIV expression in a chronically infected promonocyte clone. After acute HIV infection of U937 cells, clones that constitutively expressed varying levels of HIV were isolated by limiting dilution. One clone (U1) produced low levels of HIV but was found to increase its production 20-fold after PMA induction, as detected by reverse transcriptase or A capture. Further characterization of U1 indicated that PMA could induce cellular differentiation and maturation in the clone similar to that in uninfected U937 cells. In addition, functional studies revealed that superoxide anion production from the U1 clone was not different from that of uninfected U937 cells. Electron microscopic studies of U1 indicated that PMA induced endocytotic vesicles containing many HIV particles. These studies provide a model at the clonal level to 1) examine latency or chronicity of HIV infection in monocytes and 2) delineate the signals required for conversion to high level viral expression.