Surfactant reduction of cerebral infarct size and behavioral deficit in a rat model of cerebrovascular arterial gas embolism.
Surfactant reduction of cerebral infarct size and behavioral deficit in a rat model of cerebrovascular arterial gas embolism.
复制标题
表面活性剂减少脑血管动脉气体栓塞大鼠模型中的脑梗塞面积和行为缺陷。
DOI：
10.1152/japplphysiol.01382.2012
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Armstead,StephenC
中科院分区：
文献类型：
--
作者：
Eckmann,DavidM;Armstead,StephenC
气体栓塞症常见于心脏血管外科和减压病。本研究的目的是建立一种新的体内大鼠脑血管气体栓塞模型，并利用该模型确定外源性表面活性物质对所致脑梗塞体积和伴随的长期神经功能障碍的影响。建立SD大鼠单侧脑动脉气体栓塞模型，包括静脉注射Pluronic F-127(PF-127)和Oxycell PerflouroCarbon表面活性物质预处理组。分别于栓塞后24小时和72小时行磁共振成像(MRI)检查，测定脑梗塞体积。术后29d进行抬高身体摆动试验、肢体摆放试验、本体感觉前肢和后肢试验、触须试验和Morris水迷宫试验，以评定患者的运动行为、躯体感觉障碍和空间认知功能。建立稳定的卒中模型，MRI检查显示同侧大脑半球有梗塞。GAS栓塞组大鼠有显著的认知和感觉运动障碍，包括Morris水迷宫潜伏期时间增加约三倍，EBST操作中∼20%的左侧偏向，本体感觉和胡须触觉测试中0.5%至1.5分的平均分数上升，以及肢体放置试验中的3.0点(平均)上升，所有这些都在栓塞后期持续存在。使用PF-127或Oxycell的表面活性物质预防中风，使MRI扫描无法检测到中风，并显著减少了栓塞后大鼠认知和感觉运动能力的缺陷，EBST和胡须触觉测试在7天内恢复正常。
Gas embolism occurs commonly in cardiac and vascular surgery and decompression sickness. The goals of this study were to develop a new in vivo rat model of cerebrovascular arterial gas embolism and to determine the effects of exogenous surfactants on resultant brain infarct volume and accompanying long-term neurological dysfunction using the model. Unilateral cerebral arterial gas embolism was induced in Sprague Dawley rats, including groups receiving intravenous Pluronic F-127 (PF-127) and Oxycyte perflourocarbon surfactant pretreatment. Magnetic resonance imaging (MRI) was performed at 24 and 72 h postembolism to determine infarct volume. The elevated body swing test (EBST), limb-placement test, proprioception forelimb and hindlimb tests, whisker tactile test, and Morris Water Maze test were performed to assess motor behavior, somatosensory deficit, and spatial cognitive function out to 29 days after embolization. A stable stroke model was developed with MRI examination revealing infarction in the ipsilateral cerebral hemisphere. Gas embolized rats had significant cognitive and sensorimotor dysfunction, including approximately threefold increase in Morris Water Maze latency time, ∼20% left-sided biasing in EBST performance, 0.5 to 1.5 (mean) point score elevations in the proprioception and whisker tactile tests, and 3.0 point (mean) elevation in the limb-placement test, all of which were persistent throughout the postembolic period. Surfactant prophylaxis with either PF-127 or Oxycyte rendered stroke undetectable by MRI scanning and markedly reduced the postembolic deficits in both cognitive and sensorimotor performance in treated rats, with normalization of EBST and whisker tactile tests within 7 days.