Evaluation of in vitro Streptococcus mutans and Actinomyces naeslundii attachment and growth on restorative materials surfaces.
Evaluation of in vitro Streptococcus mutans and Actinomyces naeslundii attachment and growth on restorative materials surfaces.
复制标题
评估体外变形链球菌和内氏放线菌在修复材料表面上的附着和生长。
DOI：
10.1111/adj.12715
复制
发表时间：
2019
影响因子：
2.1
通讯作者：
M. Burrow
中科院分区：
文献类型：
--
作者：
CX Wei;W. Leung;M. Burrow
背景
修复材料具有与天然牙齿不同的表面特性，这可能会影响口腔细菌表面的附着/生长。这项研究检测了变形链球菌(Sm)或奈氏放线菌(An)在不同修复材料和牙齿表面48小时的生长情况。
方法
培养48h的Sm，观察Sm在抛光(180粒或1200粒)、唾液包被树脂复合体(RC)、玻璃离子水门汀(GIC)、树脂修饰的GIC(R-GIC)、含酪蛋白磷酸肽的GIC[3%(w/w)，CPP-ACP GIC]、银汞合金或牙块(5×5×1mm~3)上的生长情况。
结果
粗糙抛光(Ra：1.5-1.75um)材料表面显示出相对较高比例的无机、带正电的表面成分[(Si+Al)/C]和表面附着的细菌数量比光滑抛光(Ra：0.20-0.35um)材料组(p<；0.001)。与树脂基材(RC、R-GIC)和银汞合金(p≤0.003)相比，Sm和An在牙齿、光滑抛光的GIC和CPP-ACP GIC表面观察到更少的Sm和An。Sm在银汞合金表面的活性较低(p<；0.001)，而AN在银汞合金表面和粗糙的CPP-ACP GIC表面上的活性都较低(p≤0.033)。
结论
表面粗糙度对细菌的体外生长/附着的Sm/An量有显著的影响，但可能不会影响细菌的存活。有趣的是，尽管测试的各种材料的表面更光滑，但观察到附着在牙齿表面的Sm/An较少。
BACKGROUND
Restorative materials have varying surface characteristics from natural tooth, which may affect oral-bacterial surface attachment/growth. This study examined 48-hour Streptococcus mutans (Sm) or Actinomyces naeslundii (An) growth on various restorative materials and tooth surfaces.
METHODS
The quantity and viability of 48-hour-old Sm and An growth on polished (180- or 1200-grits), saliva-coated resin composite (RC), glass ionomer cements (GIC), resin-modified GIC (R-GIC), GIC containing casein phosphopeptide-amorphous calcium phosphate [3% (w/w), CPP-ACP GIC], amalgam or tooth blocks (5×5×1mm3 ) were examined.
RESULTS
Rough-polished (Ra: 1.50-1.75µm) material surfaces revealed relatively higher proportion of inorganic, positively charged surface components [(Si+Al)/C] and greater quantity of surface attached bacteria than smooth polished (Ra: 0.20-0.35µm) material groups (p<0.001). Less Sm and An were observed on tooth, and smooth polished GIC and CPP-ACP GIC surfaces than on resin-based materials (RC, R-GIC) and amalgam (p≤0.003). Viability of Sm was found lower on amalgam surfaces (p<0.001), while that of An appeared lower on both amalgam surfaces and rough CPP-ACP GIC surfaces (p≤0.033).
CONCLUSION
Surface roughness exerted a pronounced effect on in vitro growth/attached Sm/An quantity but may not have impact on bacteria viability. Interestingly, despite smoother surfaces of various materials tested, fewer Sm/An were observed attaching on tooth surfaces.