Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells.
Interferon-gamma improves impaired dentinogenic and immunosuppressive functions of irreversible pulpitis-derived human dental pulp stem cells.
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DOI：
10.1038/srep19286
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发表时间：
2016-01-18
影响因子：
4.6
通讯作者：
Yamaza T
中科院分区：
文献类型：
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作者：
Sonoda S;Yamaza H;Ma L;Tanaka Y;Tomoda E;Aijima R;Nonaka K;Kukita T;Shi S;Nishimura F;Yamaza T
临床上，不可逆性牙髓炎的治疗方法是完全切除牙髓组织，然后用人工材料代替。人牙髓干细胞（DPSCs）自体移植在牙髓治疗中具有很高的应用潜力。从健康牙齿中分离的DPSCs的用途是有限的。然而，从不可逆牙髓炎患病牙齿中分离的DPSCs （IP-DPSCs）被认为适合用于牙本质/牙髓再生。在本研究中，我们检测了IP-DPSCs的干细胞效力。与健康的DPSCs相比，IP-DPSCs表达的集落形成能力、群体倍增率、细胞增殖、多能性、体内牙本质再生和免疫抑制活性较低，这表明完整的IP-DPSCs可能不适合牙本质/牙髓再生。因此，我们试图改善IP-DPSCs体内受损的牙本质再生和体外免疫抑制功能，以实现牙本质/牙髓再生。干扰素γ (IFN-γ)处理增强了体内牙本质再生和体外T细胞抑制IP-DPSCs，而肿瘤坏死因子α处理则没有。因此，这些发现表明IFN-γ可能是一种可行的调节剂，可以改善受损的IP-DPSCs的功能，这表明IFN-γ加速的IP-DPSCs的自体移植可能是未来牙本质/髓组织工程治疗中有前景的新治疗策略。
Clinically, irreversible pulpitis is treated by the complete removal of pulp tissue followed by replacement with artificial materials. There is considered to be a high potential for autologous transplantation of human dental pulp stem cells (DPSCs) in endodontic treatment. The usefulness of DPSCs isolated from healthy teeth is limited. However, DPSCs isolated from diseased teeth with irreversible pulpitis (IP-DPSCs) are considered to be suitable for dentin/pulp regeneration. In this study, we examined the stem cell potency of IP-DPSCs. In comparison with healthy DPSCs, IP-DPSCs expressed lower colony-forming capacity, population-doubling rate, cell proliferation, multipotency, in vivo dentin regeneration, and immunosuppressive activity, suggesting that intact IP-DPSCs may be inadequate for dentin/pulp regeneration. Therefore, we attempted to improve the impaired in vivo dentin regeneration and in vitro immunosuppressive functions of IP-DPSCs to enable dentin/pulp regeneration. Interferon gamma (IFN-γ) treatment enhanced in vivo dentin regeneration and in vitro T cell suppression of IP-DPSCs, whereas treatment with tumor necrosis factor alpha did not. Therefore, these findings suggest that IFN-γ may be a feasible modulator to improve the functions of impaired IP-DPSCs, suggesting that autologous transplantation of IFN-γ-accelerated IP-DPSCs might be a promising new therapeutic strategy for dentin/pulp tissue engineering in future endodontic treatment.