The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection
The sialylation of bronchial mucins secreted by patients suffering from cystic fibrosis or from chronic bronchitis is related to the severity of airway infection
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DOI：
10.1093/glycob/9.3.311
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发表时间：
1999-03-01
期刊：
影响因子：
4.3
通讯作者：
Roussel, P
中科院分区：
文献类型：
--
作者：
Davril, M;Degroote, S;Roussel, P
从14例囊性纤维化患者和24例慢性支气管炎患者的痰中提纯粘蛋白，用两次CsBR密度梯度离心机，以每个分泌物中DNA含量作为判断感染严重程度的指标，将粘蛋白分为4组，分别为囊性纤维化感染组和非感染组，慢性支气管炎感染组和非感染组。所有囊性纤维化患者均由铜绿假单胞菌定植。正如已经观察到的，囊性纤维化患者的粘蛋白含量高于慢性支气管炎患者的粘蛋白含量。然而，与非感染患者相比，严重感染患者分泌的粘液中唾液酸含量显著增加。在38例支气管粘蛋白中有36例含有唾液酸化Lewis x表位，该表位甚至在Lewis阴性的受试者中表达，表明至少有一种不同于Lewis酶的α-1，3岩藻糖基转移酶参与了该表位的生物合成，最后，在严重感染患者的粘蛋白中也过表达了sialyl-Lewis x表位。总之，感染和未感染患者粘蛋白糖基化过程的这些差异表明，细菌感染影响人支气管粘膜唾液酸基转移酶和α1，3岩藻糖基转移酶的表达。
Bronchial mucins were purified from the sputum of 14 patients suffering from cystic fibrosis and 24 patients suffering from chronic bronchitis, using two CsBr density-gradient centrifugations, The presence of DNA in each secretion was used as an index to estimate the severity of infection and allowed to subdivide the mucins into four groups corresponding to infected or noninfected patients with cystic fibrosis, and to infected or noninfected patients with chronic bronchitis. All infected patients suffering from cystic fibrosis were colonized by Pseudomonas aeruginosa. As already observed, the mucins from the patients with cystic fibrosis had a higher sulfate content than the mucins from the patients with chronic bronchitis. However, there was a striking increase in the sialic acid content of the mucins secreted by severely infected patients as compared to noninfected patients. Thirty-six bronchial mucins out of 38 contained the sialyl-Lewis x epitope which was even expressed by subjects phenotyped as Lewis negative, indicating that at least one alpha 1,3 fucosyltransferase different from the Lewis enzyme was involved in the biosynthesis of this epitope, Finally, the sialyl-Lewis x determinant was also overexpressed in the mucins from severely infected patients. Altogether these differences in the glycosylation process of mucins from infected and noninfected patients suggest that bacterial infection influences the expression of sialyltransferases and alpha 1,3 fucosyltransferases ire the human bronchial mucosa.