Chiral separation of hesperidin and naringin and its analysis in a butanol extract of Launeae arborescens
Chiral separation of hesperidin and naringin and its analysis in a butanol extract of Launeae arborescens
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DOI：
10.1080/14786410903178376
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发表时间：
2010-01-01
影响因子：
2.2
通讯作者：
Vanthuyne, Nicolas
中科院分区：
文献类型：
--
作者：
Belboukhari, Nasser;Cheriti, Abdelkrim;Vanthuyne, Nicolas
从木犀科植物月桂地上部分分离得到两个黄酮苷类化合物，分别鉴定为橙皮苷和柚皮苷。它们是许多柑橘类水果可食用部分中含量最丰富的类黄酮类化合物。在这项研究中，我们感兴趣的是手性分离和测定橙皮苷和柚皮苷的非对映异构体势垒。采用11种手性固定相和不同的正己烷/醇流动相，在正相模式下完成了橙皮苷和柚皮苷对映异构体的高效液相色谱手性分离筛选。在Chiralpak IC和Chiralpak IA上分别测定了黄烷酮、柚皮苷和橙皮苷的非对映聚合反应的速率常数和活化能。在Chiralpak IC上用高效液相色谱法证实了木犀草丁醇提取物中分离到的黄酮类成分。
Two flavanone glycosides were isolated from the aerial part of Launeae arborescens (Asteraceae), which were identified as hesperidin and naringin. They are the most abundant flavonoids in the edible parts of many species of citrus fruits. In this study, we were interested in the chiral separation and determination of the diastereomerisation barriers of hesperidin and naringin by HPLC methods. The chiral separation HPLC screening of diastereomers of hesperidin and naringin by HPLC methods was accomplished in the normal-phase mode using 11 chiral stationary phases and various n-hexane/alcohol mobile phases. The rate constants and activation energy of diastereomerisation (G#) of flavanones, naringin and hesperidin were determined, respectively, on Chiralpak IC and Chiralpak IA. The analysis of flavanones isolated in butanol extracts of Launeae arborescens were confirmed by HPLC on Chiralpak IC.