Cell Non-autonomous Function of daf-18/PTEN in the Somatic Gonad Coordinates Somatic Gonad and Germline Development in C. elegans Dauer Larvae.
Cell Non-autonomous Function of daf-18/PTEN in the Somatic Gonad Coordinates Somatic Gonad and Germline Development in C. elegans Dauer Larvae.
复制标题
daf-18/PTEN 在体细胞性腺中的细胞非自主功能协调线虫 Dauer 幼虫的体细胞性腺和种系发育。
DOI：
10.1016/j.cub.2019.01.076
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Greenwald,Iva
中科院分区：
文献类型：
--
作者：
Tenen,ClaudiaC;Greenwald,Iva
秀丽隐杆线虫幼虫整合环境信息和发育决策[1-3]。在有利的条件下，蠕虫快速而连续地发育，经过四个幼虫阶段进入生殖成年期。然而，如果条件是不利的，通过第二幼虫阶段，蠕虫进入dauer滞育，一个全面的和可逆的发展停滞的状态，其中前体细胞保持静止和保存发展潜力，如果条件改善，预计发展进程。来自神经元、皮下组织和肠的信号调节达乌尔幼虫的外观和行为以及非性腺索马体发育停滞的许多方面[1，4，5]。在这里，我们表明，体细胞性腺母细胞（SGBs）和生殖系干细胞（GSC）的决定是静止的或发展的调节不同的非性腺索马：daf-18/PTEN的行为非自主性的体细胞性腺内，以维持两个SGBs和GSC的发展静止。我们的分析表明，daf-18在体细胞性腺细胞中起作用，产生一种（或多种）“促休眠”信号，在体细胞性腺和生殖细胞之间起作用。推断的信号不需要β 2/胰岛素受体或维持附近性成肌细胞的静止，并且发育进展indaf-18（0）不需要达法膦酸。破坏道尔的静止状态导致道尔后不育。我们的研究结果表明，体细胞性腺作为一种内分泌器官，同步体细胞性腺和生殖细胞的发育过程中dauer滞育和恢复，我们的发现，PTEN的行为非自主控制胚细胞静止可能是相关的，其功能作为一种肿瘤抑制剂在哺乳动物和打击寄生线虫。
C.eleganslarvae integrate environmental information and developmental decisions [1–3]. In favorable conditions, worms develop rapidly and continuously through four larval stages into reproductive adulthood. However, if conditions are unfavorable through the second larval stage, worms enter dauer diapause, a state of global and reversible developmental arrest in which precursor cells remain quiescent and preserve developmental potential, anticipating developmental progression if conditions improve. Signaling from neurons, hypodermis, and intestine regulate the appearance and behavior of dauer larvae and many aspects of developmental arrest of the non-gonadal soma [1, 4, 5]. Here, we show that the decision of somatic gonad blast cells (SGBs) and germline stem cells (GSCs) to be quiescent or progress developmentally is regulated differently from the non-gonadal soma:daf-18/PTEN acts non-autonomously within the somatic gonad to maintain developmental quiescence of both SGBs and GSCs. Our analysis suggests thatdaf-18acts in somatic gonad cells to produce a "pro-quiescence" signal (or signals) that actsinter seand between the somatic gonad and the germline. The inferred signal does not require DAF-2/insulin receptor or maintain quiescence of the nearby sex myoblasts, and developmental progression indaf-18(0)does not require dafachronic acids. Abrogating quiescence in dauer results in post-dauer sterility. Our results implicate the somatic gonad as an endocrine organ to synchronize somatic gonad and germline development during dauer diapause and recovery, and our finding that PTEN acts non-autonomously to control blast cell quiescence may be relevant to its function as a tumor suppressor in mammals and to combating parasitic nematodes.