Effect of magnesium ion concentration on two-dimensional structure of DNA-functionalized nanoparticles on supported lipid bilayer
Effect of magnesium ion concentration on two-dimensional structure of DNA-functionalized nanoparticles on supported lipid bilayer
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DOI：
10.7567/jjap.55.03df11
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发表时间：
2016-03-01
影响因子：
1.5
通讯作者：
Tagawa, Miho
中科院分区：
文献类型：
--
作者：
Isogai, Takumi;Akada, Eri;Tagawa, Miho
研究了二价阳离子对脂质双层辅助 DNA 功能化纳米颗粒 (DNA-NP) 组装的影响。我们之前报道了由于脂质分子在支持的脂质双层（SLB）中的移动性，DNA-NP在平面脂质双层斑块上的横向扩散，以及由此产生的DNA-NP的二维（2D）组装。我们在这里报告了在成功形成的均匀 SLB 上通过镁离子浓度控制组装的 2D DNA-NP 晶格的结构变化。在无镁缓冲溶液中，SLB 上的 DNA-NP 松散地组装成准六方有序晶格。在含有1mM醋酸镁的缓冲溶液中，DNA-NPs的颗粒间距离减小，晶格结构变得无序。在含有5mM醋酸镁的缓冲溶液中，DNA-NP阵列的结构发生明显变化，出现方格。研究表明，镁离子会影响连接纳米颗粒的 DNA 分子，从而能够控制 DNA-NP 2D 阵列的结构。 (C) 2016 日本应用物理学会
The effect of divalent cations on lipid-bilayer-assisted DNA-functionalized nanoparticle (DNA-NP) assembly has been studied. We previously reported the lateral diffusion of DNA-NPs on planar lipid bilayer patches, owing to the mobility of lipid molecules in a supported lipid bilayer (SLB), and the resultant two-dimensional (2D) assembly of DNA-NPs. We here report the structural change of the assembled 2D DNA-NP lattices by magnesium ion concentration control on a successfully formed uniform SLB. In the magnesium-free buffer solution, DNA-NPs on SLB loosely assembled into quasi-hexagonal ordered lattices. In buffer solution containing 1mM magnesium acetate, the interparticle distance of DNA-NPs decreased and the lattice structure became disordered. In buffer solution containing 5mM magnesium acetate, the structure of DNA-NP arrays changed markedly and square lattices appeared. It is suggested that magnesium ions affected DNA molecules, which linked nanoparticles, and enabled the control of the structure of DNA-NP 2D arrays. (C) 2016 The Japan Society of Applied Physics