Defining murine monocyte differentiation into colonic and ileal macrophages
Defining murine monocyte differentiation into colonic and ileal macrophages
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DOI：
10.7554/elife.49998
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发表时间：
2020-01-08
期刊：
影响因子：
7.7
通讯作者：
Jung, Steffen
中科院分区：
文献类型：
--
作者：
Gross-Vered, Mor;Trzebanski, Sebastien;Jung, Steffen
单核细胞是循环的短命巨噬细胞前体，根据需要从血液中募集到炎症和攻击部位。在稳定状态下，经典单核细胞会产生血管驻留细胞，这些细胞在内皮的管腔侧巡逻。此外，经典单核细胞为选定器官的巨噬细胞区室提供营养，包括皮肤和肠道等屏障组织以及心脏。炎症条件下的单核细胞分化已得到相当详细的研究。相比之下，非炎症条件下的单核细胞分化仍然不太清楚。在这里，我们利用细胞消融和前体植入的结合来研究单核细胞产生肠道巨噬细胞。总的来说，我们确定了与单核细胞逐渐适应组织驻留相关的因素。此外，对单核细胞分化为结肠和回肠巨噬细胞的比较揭示了肠道片段特异性基因表达特征的逐渐获得。
Monocytes are circulating short-lived macrophage precursors that are recruited on demand from the blood to sites of inflammation and challenge. In steady state, classical monocytes give rise to vasculature-resident cells that patrol the luminal side of the endothelium. In addition, classical monocytes feed macrophage compartments of selected organs, including barrier tissues, such as the skin and intestine, as well as the heart. Monocyte differentiation under conditions of inflammation has been studied in considerable detail. In contrast, monocyte differentiation under non-inflammatory conditions remains less well understood. Here we took advantage of a combination of cell ablation and precursor engraftment to investigate the generation of gut macrophages from monocytes. Collectively, we identify factors associated with the gradual adaptation of monocytes to tissue residency. Moreover, comparison of monocyte differentiation into the colon and ileum-resident macrophages revealed the graduated acquisition of gut segment-specific gene expression signatures.