Transforming growth factor-β-inducible gene-h3 (βig-h3) promotes cell adhesion of human astrocytoma cells in vitro:: implication of α6β4 integrin
Transforming growth factor-β-inducible gene-h3 (βig-h3) promotes cell adhesion of human astrocytoma cells in vitro:: implication of α6β4 integrin
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DOI：
10.1016/s0304-3940(02)01260-0
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发表时间：
2003-01-16
影响因子：
2.5
通讯作者：
Lee, EH
中科院分区：
文献类型：
--
作者：
Kim, MO;Yun, SJ;Lee, EH
big-h3 is a secretory protein that is induced by transforming growth factor (TGF)-beta. We have recently found that betaig-h3 expression is induced in cultured astrocytes by TGF-beta1 and in rat cerebral cortex by stab wound. The purpose of this study was to examine the effect of the secreted betaig-h3 on cell adhesion of astrocytes and the underlying mechanisms. When U87 human astrocytoma cells were seeded on dishes coated with recombinant betaig-h3, cell adhesion was significantly enhanced. Blocking experiments using various antibodies to the integrin subunit suggested alpha6beta4 integrin could be involved in the betaig-h3-mediated astrocyte cell adhesion. Cell adhesion to betaig-h3 substrate was substantially blocked by preincubation with the inhibitor to the src kinase. When cells were plated on betaig-h3-coated dishes, tyrosine phosphorylation of focal adhesion kinase was prominently increased within 20 min in a beta4 integrin-dependent manner. The results suggest that alpha6beta4 integrin-mediated interactions of astrocytes with betaig-h3 transduce intracellular signals through the focal adhesion proteins, which may regulate certain aspects of astrocyte response to brain injury. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.
