Retinal vessel oximetry-calibration, compensation for vessel diameter and fundus pigmentation, and reproducibility
Retinal vessel oximetry-calibration, compensation for vessel diameter and fundus pigmentation, and reproducibility
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DOI：
10.1117/1.2976032
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发表时间：
2008-09-01
影响因子：
3.5
通讯作者：
Schweitzer, Dietrich
中科院分区：
文献类型：
--
作者：
Hammer, Martin;Vilser, Walthard;Schweitzer, Dietrich
本研究的目的是测量视网膜血管中血红蛋白氧合，并评估测量的灵敏度和重复性。使用配备有特殊的双波长透射滤光片的眼底照相机和彩色电荷耦合器件照相机，同时记录548和610 nm处的两个单色眼底图像。计算两种波长的视网膜血管的光密度及其比值，已知其与氧饱和度成比例。从50度的图像，使用的半自动血管识别和跟踪算法识别和测量血管直径为100 μ m或更大。平均而言，动脉和静脉血氧饱和度分别为98 +/- 10.1%和65 +/-11.7%。对于来自每个受试者的五个图像的相同血管段中的测量，在动脉和静脉中分别发现2.52%和3.25%的氧饱和度的标准差。100%氧呼吸使平均动脉和静脉血氧饱和度分别增加2%和7%。介绍了一种简单的无创光学血氧测量系统，该系统由眼底照相机中的特殊滤光片和软件组成。它能够测量视网膜分支血管中的氧饱和度，具有适用于临床研究的重复性和灵敏度。(C)2008年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI 10.1117/1.2976032]
The purpose of this study was to measure the hemoglobin oxygenation in retinal vessels and to evaluate the sensitivity and reproducibility of the measurement. Using a fundus camera equipped with a special dual wavelength transmission filter and a color charge-coupled device camera, two monochromatic fundus images at 548 and 610 nm were recorded simultaneously. The optical densities of retinal vessels for both wavelengths and their ratio, which is known to be proportional to the oxygen saturation, were calculated. From 50-deg images, the used semiautomatic vessel recognition and tracking algorithm recognized and measured vessels of 100 mu m or more in diameter. On average, arterial and venous oxygen saturations were measured at 98 +/- 10.1% and 65 +/- 11.7%, respectively. For measurements in the same vessel segments from the five images per subject, standard deviations of 2.52% and 3.25% oxygen saturation were found in arteries and veins, respectively. Respiration of 100% oxygen increased the mean arterial and venous oxygen saturation by 2% and 7% respectively. A simple system for noninvasive optical oximetry, consisting of a special filter in a fundus camera and software, was introduced. It is able to measure the oxygen saturation in retinal branch vessels with reproducibility and sensitivity suitable for clinical investigations. (C) 2008 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.2976032]