INDUCTION OF TOLERANCE TO HYPOTHERMIA AND HYPERTHERMIA BY A COMMON MECHANISM IN MAMMALIAN-CELLS
INDUCTION OF TOLERANCE TO HYPOTHERMIA AND HYPERTHERMIA BY A COMMON MECHANISM IN MAMMALIAN-CELLS
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DOI：
10.1002/jcp.1041560115
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发表时间：
1993-07-01
影响因子：
5.6
通讯作者：
KRUUV, J
中科院分区：
文献类型：
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作者：
GLOFCHESKI, DJ;BORRELLI, MJ;KRUUV, J
低温预处理通过方法4进行的附着指数相V79中国仓鼠细胞的（25 ℃）循环（PHC）（25 ℃下24小时+37 ℃下1.5小时+25 ℃下24小时+胰蛋白酶+37 ℃下3小时）或通过方法3（25 ℃ 48小时+胰蛋白酶+37 ℃ 3小时）使哺乳动物V79细胞对43 ℃的高温具有显著更强的抗性。无论方法3或4用于预暴露，43 ℃曲线均无显著差异。如果预暴露在15或10摄氏度，对高温的抵抗力会显著降低。通过方法4的PHC显著增加暴露于5 ℃的细胞的存活，并且在较小程度上增加暴露于10 ℃的细胞的存活。肝癌后高低温存活率的增加不能用细胞周期分布的变化来解释。热休克蛋白的合成不是由PHC诱导的，因此，新合成的蛋白质不会产生保护作用。当通过在37 ℃下在2%DMSO中预处理24小时使细胞耐受高温时（方法8），细胞对随后暴露于低温（5 ℃或10 ℃）没有更大的耐受性。结果表明，可能有两种机制诱导抵抗高温，只有其中之一也赋予抵抗低温。(C)1993 Wiley-Liss，Inc.
Pretreatment by hypothermic (25-degrees-C) cycling (PHC) of attached exponential-phase V79 Chinese hamster cells by Method 4(24 hr at 25-degrees-C + 1.5 hr at 37-degrees-C + 24 hr at 25-degrees-C + trypsin + 3 hr at 37-degrees-C) or by Method 3 (48 hr at 25-degrees-C + trypsin + 3 hr at 37-degrees-C) make mammalian V79 cells significantly more resistant to 43-degrees-C hyperthermia. There is no significant difference in the 43-degrees-C curves whether Method 3 or 4 is used for pre-exposure. If pre-exposure is at 15 or 10-degrees-C, the resistance to hyperthermia is significantly reduced. PHC by Method 4 significantly increases survival of cells exposed to 5-degrees-C and, to a lesser extent, to 10-degrees-C. The increase in hyper- and hypothermic survival after PHC cannot be accounted for by changes in cell cycle distribution. Heat-shock protein synthesis is not induced by PHC; hence, protection does not result from newly synthesized proteins. When cells are made tolerant to hyperthermia by a pretreatment in 2% DMSO for 24 hr at 37-degrees-C (Method 8), the cells are not more resistant to subsequent exposures to hypothermia, either at 5 or 10-degrees-C. The results imply that there may be two mechanisms of inducing resistance to hyperthermia, only one of which also confers resistance to hypothermia. (C) 1993 Wiley-Liss, Inc.