Combined NMR and MS-based metabonomics and real-time PCR analyses reveal dynamic metabolic changes of Ganoderma lucidum during fruiting body growing
Combined NMR and MS-based metabonomics and real-time PCR analyses reveal dynamic metabolic changes of Ganoderma lucidum during fruiting body growing
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DOI：
10.1016/j.foodres.2024.114056
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发表时间：
2024-02-08
影响因子：
8.1
通讯作者：
Liu，Maili
中科院分区：
文献类型：
--
作者：
Liu，Caixiang;Chen，Fangfang;Liu，Maili
灵芝是一种珍稀药用真菌，具有多种药用价值。灵芝酸(GAS)是其主要成分之一，是一种重要的三萜类化合物，具有镇静止痛、保肝、抗肿瘤等作用。了解中国企业的成长和发展。发光子实体是决定采收它们的最佳时间的关键。在本研究中，我们使用核磁共振波谱对G的代谢物进行了系统的表征。在七个不同的发育阶段。用LC-MS/MS测定了7种气体的含量，共检出49种代谢物。灵芝，包括氨基酸、糖、有机酸和气体。在从芽发育期(I)到现蕾期(II)的过渡过程中，我们观察到葡萄糖、酪氨酸、烟酰胺核苷、肌苷和GAs的快速积累。现蕾期以后，大部分代谢产物含量下降，直到成熟期(Vii)。除GAF含量在成熟期迅速升高外，GAs含量在拔节期呈先升高后下降的趋势。我们还检测了几个参与GA合成的基因的表达，发现包括16个细胞色素P450单加氧酶在内的大多数基因在IV和VII期都比I期下调。这些发现为了解G的动态代谢谱提供了有价值的见解。在其整个生长阶段都有光泽，建议收获。Lucidumat期IV。
Ganoderma lucidum(G. lucidum) is a rare medicinal fungus with various beneficial properties. One of its main components, ganoderic acids (GAs), are important triterpenoids known for their sedative and analgesic, hepatoprotective, and anti-tumor activities. Understanding the growth and development of theG. lucidumfruiting body is crucial for determining the optimal time to harvest them. In this study, we used nuclear magnetic resonance (NMR) spectroscopy to systematically characterize the metabolites ofG. lucidumat seven distinct developmental stages. We also measured the contents of seven kinds of GAs using LC-MS/MS. A total of 49 metabolites were detected inG. lucidum, including amino acids, sugars, organic acids and GAs. During the transition from the bud development period (I) to the budding period (II), we observed a rapid accumulation of glucose, tyrosine, nicotinamide ribotide, inosine and GAs. After the budding period, the contents of most metabolites decreased until the mature period (VII). In addition, the contents of GAs showed an initial raising, followed by a decline during the elongation period, except for GAF, which exhibited a rapid raise during the mature stage. We also detected the expression of several genes involved in GA synthesis, finding that most genes including 16 cytochromeP450monooxygenase were all down-regulated during periods IV and VII compared to period I. These findings provide valuable insights into the dynamic metabolic profiles ofG. lucidumthroughout its growth stage, and it is recommended to harvestG. lucidumat period IV.