Resumption of prolactin secretion after dopaminergic inhibition: differential effects of dopamine and its agonists.
Resumption of prolactin secretion after dopaminergic inhibition: differential effects of dopamine and its agonists.
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多巴胺能抑制后催乳素分泌的恢复：多巴胺及其激动剂的不同作用。
DOI：
10.1152/ajpendo.1981.240.6.e700
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
P. Woolf
中科院分区：
文献类型：
--
作者：
P. Woolf
采用垂体单层培养法研究了多巴胺能抑制后催乳素分泌的恢复情况。10(-6) M多巴胺（DA）、10(-7)M阿波啡（APO）和10(-10)M溴隐亭（分别为45%、42%和51%）抑制4 h内PRL分泌，细胞内激素含量相互增加。去药后，经DA处理的培养物在第2 h的PRL分泌率为对照的208%，但在4 h后恢复到对照水平，而APO处理后的PRL分泌率在4 h的恢复期为对照的131-142%。相比之下，去除溴隐亭20 h后，PRL的分泌量仍显著低于对照组。虽然氟哌啶醇（10(-7)M）在同时添加时可以阻止溴隐亭的抑制作用，但在治疗后添加氟哌啶醇并不能恢复PRL的分泌。因此，DA和APO抑制PRL释放是容易可逆的，随后是早期PRL高分泌。然而，溴隐亭的作用是持续的，一旦开始就不会被DA拮抗剂逆转。
The recovery of prolactin (PRL) secretion following dopaminergic inhibition was studied in vitro using pituitary monolayer cultures. PRL secretion over 4 h was inhibited comparably by 10(-6) M dopamine (DA), 10(-7) M apomorphine (APO), and 10(-10) M bromocriptine (45%, 42%, 51%, respectively) with reciprocal increases in intracellular hormone content. After drug removal, the PRL secretion rate in the cultures that had been treated with DA was 208% of the control cultures by the 2nd h but returned to control values by 4 h, whereas the secretion rate after APO was 131-142% of control throughout the 4-h recovery period. In contrast, PRL secretion remained significantly less than control 20 h after the removal of bromocriptine. Although haloperidol (10(-7) M) prevented the inhibitory action of bromocriptine when added simultaneously, the addition of haloperidol did not restore PRL secretion when added in the posttreatment period. Thus, DA and APO inhibition of PRL release is readily reversible and is followed by early PRL hypersecretion. However, bromocriptine's effects are sustained and once started are not reversed by DA antagonists.