Staining and fluorescent microscopy methods for pollen viability determination in sunflower and other plant species
Staining and fluorescent microscopy methods for pollen viability determination in sunflower and other plant species
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DOI：
10.1016/j.indcrop.2011.06.012
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发表时间：
2012-01-01
影响因子：
5.9
通讯作者：
Marjanovic-Jeromela, Ana
中科院分区：
文献类型：
--
作者：
Atlagic, Jovanka;Terzic, Sreten;Marjanovic-Jeromela, Ana
本文的结果表明Alexander（1969）的染色方法的成功应用，该方法与原始方法相比简化了，并适用于向日葵花粉活力的估计。油菜籽、甜菜、玉米、小麦、苜蓿等。描述了经常使用这种方法的研究方面。本文还介绍了荧光显微法（Kho and Baer, 1968）在向日葵花粉萌发和花粉管生长监测中的应用结果，以及荧光显微法在施肥潜力估算中的应用可能性。(C) 2011 Elsevier B.V.版权所有
Results shown in this paper imply a successful application of staining method by Alexander (1969) which is simplified in comparison to the original method and adapted for pollen viability estimation in sunflower. rapeseed, sugar beet, maize, wheat, alfalfa, etc. Aspects of research in which this method is frequently used are described.This paper also shows the results of fluorescent microscopy method (Kho and Baer, 1968) adaptation for pollen germination and pollen tube growth monitoring in sunflower, as well the possibility for its application in fertilization potential estimation. (C) 2011 Elsevier B.V. All rights reserved.