[Study on the effects of total flavonoids from litchi nucleus on nuclear translocation of nuclear factor-kappa B and related protein expression in rat hepatic stellate cell].
[Study on the effects of total flavonoids from litchi nucleus on nuclear translocation of nuclear factor-kappa B and related protein expression in rat hepatic stellate cell].
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DOI：
10.3760/cma.j.issn.1007-3418.2018.07.011
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发表时间：
2018-07-20
影响因子：
--
通讯作者：
Lu, Q
中科院分区：
文献类型：
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作者：
Qin, G J;Zhao, Y Z;Lu, Q
目的：研究荔枝总黄酮（TFL）对体外转化生长因子-β1（TGF-β1）诱导的大鼠肝星状细胞系（HSC-T6）核转位的影响，探讨抗肝纤维化药物的作用机制。方法：体外培养HSC-T6，TGFβ1诱导24 h，然后用125、250和500 μg/ml TFL处理48 h。通过共聚焦激光显微镜观察TFL对HSC-T6中NF-κB核转位的影响。 Western blot检测TFL对TLR4、p-IkappaBɑ、p-NF-kappaB p65、NF-kappaB和Collagen I蛋白表达的影响。免疫荧光法检测TLR4和p-NF-kappaB p65的表达。数据表示为平均值±SEM。进行方差齐性检验，然后进行单向方差分析（ANOVA）。组间多重比较采用LSD检验。 P < 0.05 被认为具有统计学意义。结果：共聚焦激光扫描显微镜显示，TFL以浓度依赖性方式抑制活化的HSC-T6中NF-kappaB的核转位，并以浓度依赖性方式下调HSC-T6中TLR4、p-IkappaBɑ、p-NF-kappaB p65、NF-kappaB和I型胶原蛋白的蛋白表达水平。结论:TFL抗肝纤维化的作用机制可能与抑制活化的HSC-T6中NF-kappab核转位以及HSC-T6中TLR4、P-ikappabɑ、P-nf-kappab p65、NF-kappab和I型胶原蛋白的表达有关。
Objective: The effect of total flavonoids of litchi (TFL) on nuclear translocation of nuclear factor-kappa B (NF- kappa B) in rat hepatic stellate cell line (HSC-T6) induced by transforming growth factor - beta 1 (TGF- beta 1) in vitro was studied to explore the mechanism of action of anti-hepatic fibrosis drugs. Methods: HSC-T6 was cultured in vitro, induced by TGFbeta1 for 24 h, and then treated with TFL at 125, 250 and 500 mug/ml for 48 h. The effect of TFL on NF-kappaB nuclear translocation in HSC-T6 was observed by confocal laser microscopy. The effects of TFL on the expression of TLR4, p-IkappaB ɑ, p-NF-kappaB p65, NF-kappaB and Collagen I protein were detected by western blot. The expressions of TLR4 and p-NF-kappaB p65 were detected by immunofluorescence. Data were presented as mean±SEM. Homogeneity test of variance was performed and then followed by one-way analysis of variance (ANOVA). The multiple comparisons between groups were performed by LSD test. P < 0.05 was considered statistically significant. Results: Confocal laser scanning microscopy showed TFL inhibited the nuclear translocation of NF-kappaB in activated HSC-T6 in a concentration-dependent manner and TFL down regulated the protein expression levels of TLR4, p-IkappaB ɑ, p-NF-kappaB p65, NF-kappaB and collagen I protein in HSC-T6 in a concentration-dependent manner. Conclusion: The mechanism of TFL against hepatic fibrosis may be related to the inhibition of nuclear translocation of NF-kappab in the activated HSC-T6 and the expression of TLR4, P-ikappabɑ, P-nf-kappab p65, NF-kappab and collagen I protein in HSC-T6.