Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared.
Measurement of cyclosporine concentrations in whole blood: HPLC and radioimmunoassay with a specific monoclonal antibody and 3H- or 125I-labeled ligand compared.
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全血中环孢菌素浓度的测量：用特定单克隆抗体和 3 H 或 125 I 标记配体与 HPLC 和放射免疫测定法进行比较。
DOI：
10.1093/clinchem/35.1.120
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发表时间：
1989
影响因子：
9.3
通讯作者：
M. Scott
中科院分区：
文献类型：
--
作者：
B. Wolf;M. Daft;J. Koenig;M. Flye;J. Turk;M. Scott
我们比较了用~3H或~(125)I标记环孢素配体的环孢素单抗与高效液相色谱法和放射免疫法测定的环孢素在全血中的浓度。与高效液相色谱法相比，~3H-RIA试剂盒略微低估了环孢素浓度(大于600微克/L)。在广泛的浓度范围内，用125I-RIA试剂盒测量的环孢素与高效液相色谱相关性良好(斜率=0.99，n=301，r=0.98)，观察到来自肾、心脏或肝脏移植受者的样本(斜率分别为1.01、0.93和1.00)。125I-RIA标准曲线与每升1000微克环孢素呈线性关系。125I-RIA测定环孢素的批间和批内变异系数均小于或等于7%。因此，125I-RIA试剂盒与高效液相色谱法相当，可替代125I-RIA试剂盒用于药物监测。
We compared cyclosporine concentrations in whole blood as measured by HPLC and by RIA with a monoclonal antibody specific for cyclosporine with 3H- or 125I-labeled cyclosporine ligand. The 3H-RIA kit slightly underestimated cyclosporine concentrations (greater than 600 micrograms/L) in comparison with HPLC. Over a wide range of concentrations, cyclosporine measured with the 125I-RIA kit correlated well with HPLC (slope = 0.99, n = 301, r = 0.98), observed for samples from recipients of kidney, heart, or liver allografts (respective slopes: 1.01, 0.93, and 1.00). The 125I-RIA standard curve was linear to 1000 micrograms of cyclosporine per liter. Inter- and intra-assay CVs for 125I-RIA measurements of cyclosporine were less than or equal to 7%. Evidently, the 125I-RIA kit involving a monoclonal antibody specific for cyclosporine is equivalent to the HPLC assay and can replace it for therapeutic drug monitoring of cyclosporine therapy.