The fetal rat binding protein for insulin-like growth factors is expressed in the choroid plexus and cerebrospinal fluid of adult rats.
The fetal rat binding protein for insulin-like growth factors is expressed in the choroid plexus and cerebrospinal fluid of adult rats.
复制标题
胎鼠胰岛素样生长因子结合蛋白在成年大鼠的脉络丛和脑脊液中表达。
DOI：
10.1210/mend-3-10-1559
复制
发表时间：
1989
影响因子：
--
通讯作者：
M. Rechler
中科院分区：
文献类型：
--
作者：
L. Y. Tseng;Alexandra L. Brown;Yvonne W.;J. A. Romanus;C. Orlowski;T. Taylor;M. Rechler
胰岛素样生长因子IGF-I和IGF-II对其受体的生物学作用是由igf结合蛋白调节的。最近，我们分离到了igf结合蛋白家族成员BP-3A的cDNA克隆，这是一个由BRL-3A大鼠肝细胞系合成的30千道尔顿（kDa）蛋白。BP-3A与另外两种克隆的igf结合蛋白（人羊水结合蛋白和血清中150 kDa igf结合蛋白复合物的糖基化结合亚基）相关但又不同。它在胎鼠的多个非神经组织和血清中表达，出生后减少，与IGF-II表达的发育模式相似。igf - 1、igf - 2及其受体在大脑中表达。本研究检测BP-3A在大鼠中枢神经系统中的表达。通过Northern blot分析，BP-3A mRNA在妊娠21天大鼠的脑干、大脑皮层和下丘脑中高水平存在，与IGF-II mRNA一样，BP-3A mRNA在成年大鼠大脑中持续存在。利用35s标记的寡核苷酸（长度为48个碱基，与BP-3A编码区互补和反互补）原位杂交，在成年大鼠脑冠状切片上定位BP-3A mRNA合成位点。BP-3A探针只在脉络膜丛上特异杂交，从内侧视前核水平延伸到下丘脑弓形核，类似于先前报道的IGF-II mRNA优先定位于脉络膜丛。脉络膜丛合成BP-3A mRNA提示BP-3A可能分泌到脑脊液中。在大鼠脑脊液中发现了一种30 kDa的igf结合蛋白，该蛋白可被BP-3A抗体识别，与纯化的BP-3A一样，对IGF-I和IGF-II具有相同的亲和力。与脉络丛合成的其他转运蛋白类似，BP-3A可能促进IGF-II向脑脊液的分泌，并调节其在脑内远端部位的生物学作用。
The biological effects of the insulin-like growth factors, IGF-I and IGF-II, on their receptors are modulated by IGF-binding proteins. Recently, we isolated a cDNA clone for one member of the family of IGF-binding proteins, BP-3A, a 30 kilodalton (kDa) protein synthesized by the BRL-3A rat liver cell line. BP-3A is related to but distinct from two other cloned IGF-binding proteins, the human amniotic fluid binding protein and the glycosylated binding subunit of the 150 kDa IGF-binding protein complex in serum. It is expressed in multiple nonneural tissues and in serum in the fetal rat and decreases after birth, similar to the developmental pattern of IGF-II expression. IGF-I, IGF-II, and their receptors are expressed in brain. The present study examines the expression of BP-3A in the rat central nervous system. By Northern blot analysis, BP-3A mRNA is present at high levels in brain stem, cerebral cortex, and hypothalamus from 21-day gestation rats and, like IGF-II mRNA, persists in adult rat brain. The site of BP-3A mRNA synthesis was localized by in situ hybridization to coronal sections of adult rat brain using 35S-labeled oligonucleotides, 48 bases in length, complementary and anticomplementary to the coding region of BP-3A. Specific hybridization of the BP-3A probe was observed exclusively to the choroid plexus extending from the level of the medial preoptic nucleus to the arcuate nucleus of the hypothalamus, similar to the previously reported preferential localization of IGF-II mRNA to the choroid plexus. Synthesis of BP-3A mRNA by choroid plexus suggested that BP-3A might be secreted into the cerebrospinal fluid. A 30 kDa IGF-binding protein was demonstrated in rat cerebrospinal fluid that is recognized by antibodies to BP-3A and, like purified BP-3A, has equal affinity for IGF-I and IGF-II. By analogy with other transport proteins synthesized by the choroid plexus, BP-3A may facilitate the secretion of IGF-II to the cerebrospinal fluid and modulate its biological actions at distant sites within the brain.
登录
查看更多内容
来自人血浆的哇巴因结合蛋白。
DOI：
10.1161/01.hyp.0000027134.14160.1d
发表时间：
2002
期刊：
Hypertension (Dallas, Tex. : 1979)
影响因子：
--
作者：
Parhami-Seren,Behnaz;Haberly,Richard;Margolies,MichaelN;HaupertJr,GarnerT
通讯作者：
HaupertJr,GarnerT
DOI：
10.1073/pnas.81.8.2562
发表时间：
1984-04
影响因子：
11.1
作者：
E. Recio-Pinto;F. Lang;D. Ishii
通讯作者：
E. Recio-Pinto;F. Lang;D. Ishii
脑脊液和羊水中胰岛素样生长因子结合蛋白的结构和免疫学比较。
DOI：
10.1210/jcem-68-3-638
发表时间：
1989
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Rosenfeld,RG;Pham,H;Conover,CA;Hintz,RL;Baxter,RC
通讯作者：
Baxter,RC
大鼠胎儿和成人组织中的生长调节素-C/胰岛素样生长因子-I 和胰岛素样生长因子-II mRNA。
DOI：
--
发表时间：
1986
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Lund,PK;Moats-Staats,BM;Hynes,MA;Simmons,JG;Jansen,M;D'Ercole,AJ;VanWyk,JJ
通讯作者：
VanWyk,JJ
大鼠组织中胰岛素样生长因子 II 受体免疫反应性的分布。
影响因子：
4.8
作者：
Valentino,KL;Pham,H;Ocrant,I;Rosenfeld,RG
通讯作者：
Rosenfeld,RG