Comparison of Poly(DL-Lactide-co-Glycolide) and Polystyrene Microsphere Targeting to Intestinal M Cells
Comparison of Poly(DL-Lactide-co-Glycolide) and Polystyrene Microsphere Targeting to Intestinal M Cells
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DOI：
10.3109/10611869308996082
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发表时间：
1993-01-01
影响因子：
4.5
通讯作者：
Hirst, Barry H.
中科院分区：
文献类型：
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作者：
Jepson, Mark A.;Simmons, Nicholas L.;Hirst, Barry H.
比较了聚乳酸-乙交酯(PLG)微球和聚苯乙烯微球与兔Peyer‘s斑块的滤泡相关上皮(FAE)的相互作用。PLG微球与FAE的结合比同等尺寸的聚苯乙烯微球(直径0.5-0.6 pm)低一个数量级。虽然PLG微球不像聚苯乙烯微球那样选择性地靶向于M细胞表面，但与M细胞结合的微球中有很高比例是跨细胞转运的，导致1.5x10(4)PLG微球/mm(2)FAE的跨皮递送。这是M细胞首次直接通过上皮传递PLG微球，这对这种载体用于口服药物和疫苗的潜在用途至关重要。由于天然PLG微球不是以M细胞表面为最佳靶点，因此存在通过表面修饰来进一步提高其效力的空间。
The interaction of poly(DL-lactide-co-glycolide) (PLG) and polystyrene microspheres with the follicle-associated epithelium (FAE) of rabbit Peyer's patches was compared. Binding by PLG microspheres to the FAE was an order of magnitude lower than that of polystyrene microspheres of equivalent size (0.5-0.6 pm diameter). Although PLG microspheres are not selectively targeted to the M cell surface, as is the case with polystyrene microspheres, a high proportion of those that bind to M cells are transcytosed, resulting in the transepithelial delivery of 1.5x10(4) PLG microspheres/mm(2) FAE. This represents the first direct demonstration of transepithelial delivery of PLG microspheres by M cells, which is crucial to the potential use of such vehicles for the oral delivery of drugs and vaccines. As native PLG microspheres are not optimally targeted to the M cell surface, there is scope for the further improvement of their efficacy by surface modifications.