POLARIZED SORTING OF VIRAL GLYCOPROTEINS TO THE AXON AND DENDRITES OF HIPPOCAMPAL-NEURONS IN CULTURE
POLARIZED SORTING OF VIRAL GLYCOPROTEINS TO THE AXON AND DENDRITES OF HIPPOCAMPAL-NEURONS IN CULTURE
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DOI：
10.1016/0092-8674(90)90240-f
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发表时间：
1990-07-13
期刊：
影响因子：
64.5
通讯作者：
SIMONS, K
中科院分区：
文献类型：
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作者：
DOTTI, CG;SIMONS, K
培养的海马神经元感染水泡性口炎病毒（VSV）的温度敏感的突变体和野生型株的禽流感禽瘟病毒（FPV）。通过免疫荧光显微镜监测病毒糖蛋白的细胞内分布。在成熟的，完全极化的神经元中，VSV糖蛋白（上皮MDCK细胞中的基底外侧蛋白）从高尔基复合体移动到树突状结构域，而FPV的血凝素蛋白（MDCK细胞中的顶端分选蛋白）优先靶向轴突，但不限于轴突。VSV糖蛋白呈簇状分布于树突表面，而血凝素则沿着轴突膜均匀分布。基于发现相同的病毒糖蛋白在神经元和上皮细胞中以极化方式进行分选，我们提出表面蛋白分选的分子机制在两种细胞类型中具有共同的特征。
Cultured hippocampal neurons were infected with a temperature-sensitive mutant of vesicular stomatitis virus (VSV) and a wild-type strain of the avian influenza fowl plague virus (FPV). The intracellular distribution of viral glycoproteins was monitored by immunofluorescence microscopy. In mature, fully polarized neurons the VSV glycoprotein (a basolateral protein in epithelial MDCK cells) moved from the Golgi complex to the dendritic domain, whereas the hemagglutinin protein of FPV (an apically sorted protein in MDCK cells) was targeted preferentially, but not exclusively, to the axon. The VSV glycoprotein appeared in clusters on the dendritic surface, while the hemagglutinin was distributed uniformly along the axonal membrane. Based on the finding that the same viral glycoproteins are sorted in a polarized fashion in both neuronal and epithelial cells, we propose that the molecular mechanisms of surface protein sorting share common features in the two cell types.