Serum levels of miR-29, miR-122, miR-155 and miR-192 are elevated in patients with cholangiocarcinoma
Serum levels of miR-29, miR-122, miR-155 and miR-192 are elevated in patients with cholangiocarcinoma
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DOI：
10.1371/journal.pone.0210944
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发表时间：
2019-01-17
期刊：
影响因子：
3.7
通讯作者：
Roderburg, Christoph
中科院分区：
文献类型：
--
作者：
Loosen, Sven H.;Lurje, Georg;Roderburg, Christoph
目的胆管细胞癌(CCA)是第二常见的肝脏原发恶性肿瘤。尽管开展了大量的研究活动，但大多数患者的预后仍然很差。只有在早期诊断的情况下，肝切除才有可能导致长期生存。方法采用半定量逆转录聚合酶链式反应分析94例胆管癌行肿瘤切除患者和40例健康对照血清中miR-122、miR-192、miR-29b和miR-155四种miRNA组的血清浓度。结果CCA患者血清miR-122、miR-192、miR-29b和miR-155水平明显高于健康对照组和无恶变的原发性硬化性胆管炎患者。尽管术前miRNAs水平不适合作为预后指标，但术后miR-122血清水平的显著下降与患者的预后密切相关。结论循环miRNAs分析是早期CCA的一种有前途的诊断工具。术后miRNA血清浓度的下降可能预示着患者的预后良好，有助于确定扩大肝脏手术后预后良好的患者。
ObjectivesCholangiocarcinoma (CCA) represents the second most common primary hepatic malignancy. Despite tremendous research activities, the prognosis for the majority of patients is still poor. Only in case of early diagnosis, liver resection might potentially lead to long-term survival. However, it is still unclear which patients benefit most from extensive liver surgery, highlighting the need for new diagnostic and prognostic stratification strategies.MethodsSerum concentrations of a 4 miRNA panel (miR-122, miR-192, miR-29b and miR-155) were analyzed using semi-quantitative reverse-transcriptase PCR in serum samples from 94 patients with cholangiocarcinoma undergoing tumour resection and 40 healthy controls. Results were correlated with clinical data.ResultsSerum concentrations of miR-122, miR-192, miR-29b and miR-155 were significantly elevated in patients with CCA compared to healthy controls or patients with primary sclerosing cholangitis without malignant transformation. Although preoperative levels of these miRNAs were unsuitable as a prognostic marker of survival, a strong postoperative decline of miR-122 serum levels was significantly associated with a favorable patients' prognosis.ConclusionsAnalysis of circulating miRNAs represents a promising tool for the diagnosis of even early stage CCA. A postoperative decline in miRNA serum concentrations might be indicative for a favorable patients' outcome and helpful to identify patients with a good prognosis after extended liver surgery.